Common to all fibrotic and metastatic diseases is the uncontrollable remodeling of tissue that leads to the accumulation of fibrous connective tissue components such as collagen and elastin. Build-up of fibrous tissue occurs through the cross-linking of collagen or elastin monomers, which is initiated through the oxidation of lysine residues to form α-aminoadipic-δ-semialdehyde (allysine). To provide a measure of the extent of collagen oxidation in disease models of fibrosis or metastasis, a rapid, sensitive HPLC method was developed to quantify the amount of allysine present in tissue. Allysine was reacted with sodium 2-naphthol-7-sulfonate under conditions typically applied for acid hydrolysis of tissues (6 M HCl, 110 °C, 24 h) to prepare AL-NP, a fluorescent bis-naphthol derivative of allysine. High performance liquid chromatography was applied for analysis of allysine content. Under optimal reaction and detection conditions, successful separation of AL-NP was achieved with excellent analytical performance attained. Good linear relationship (R² = 0.994) between peak area and concentration for AL-NP was attained for 0.35–175 pmol of analyte. A detection limit of 0.02 pmol in the standard sample with a 20 μL injection was achieved for AL-NP, with satisfactory recovery from 88 to 100% determined. The method was applied in the quantification of allysine in healthy and fibrotic mouse lung tissue, with the fibrotic tissue showing a 2.5 fold increase in the content of allysine.

所有纤维化和转移性疾病的共同点是组织的不可控制的重塑，导致纤维结缔组织成分（如胶原蛋白和弹性蛋白）的积累。纤维组织的积累是通过胶原蛋白或弹性蛋白单体的交联发生的，这是通过赖氨酸残基的氧化形成α-氨基己二酸-δ-半醛（赖氨酸）而引发的。为了衡量纤维化或转移性疾病模型中胶原蛋白氧化的程度，开发了一种快速，灵敏的HPLC方法来量化组织中存在的赖氨酸含量。在通常用于组织酸水解的条件下（6 M HCl，110°C，24 h），使烯丙氨酸与2-萘酚-7-磺酸钠反应，制得AL-NP，一种赖氨酸的荧光双萘酚衍生物。高效液相色谱法用于分析赖氨酸含量。在最佳的反应和检测条件下，成功分离出AL-NP，并获得了出色的分析性能。良好的线性关系（R 对于0.35–175 pmol的分析物，峰面积与AL-NP浓度之间达到² = 0.994）。进样20μL的AL-NP在标准样品中的检出限为0.02 pmol，从88％到100％的满意回收率。该方法用于健康和纤维化小鼠肺组织中的赖氨酸定量，纤维化组织的赖氨酸含量增加了2.5倍。