Fluorescein derivatives are important fluorescent probes which can be used for detection and optical imaging. Fluorescein derivatives are usually constructed by introducing aldehyde groups or esterified onto fluorescein xanthene ring and benzene moiety. Typically, the research direction of connecting amino groups with fluorescein monoaldehyde is in hot. Because of their high activity, these derivatives can be complexed with the analytes to produce changes of colors and the increase or decrease of fluorescence intensity. This article reviewed fluorescein probes in past two years according to classification of different analytes including metal ions, anions, small molecules and biological macromolecules. The synthesis methods, optical properties, possible mechanisms and applications of fluorescein probes are summarized. This article provides a reference for the screening of fluorescein probes with high sensitivity and biological detection and can propel their further applications in sensing and detection of analytes.

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荧光素作为检测分析物的荧光探针的应用

荧光素衍生物是重要的荧光探针，可用于检测和光学成像。荧光素衍生物通常是通过引入醛基或将其酯化到荧光素ring吨环和苯部分上而构建的。通常，将氨基与荧光素单醛连接的研究方向很热。由于它们的高活性，这些衍生物可以与分析物络合以产生颜色变化以及荧光强度的增加或减少。根据不同分析物（包括金属离子，阴离子，小分子和生物大分子）的分类，本文对过去两年的荧光素探针进行了综述。总结了荧光素探针的合成方法，光学性质，可能的机理和应用。