当前位置: X-MOL 学术Chem. Rev. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Structural and Chemical Biology of Terpenoid Cyclases.
Chemical Reviews ( IF 51.4 ) Pub Date : 2017-08-25 , DOI: 10.1021/acs.chemrev.7b00287
David W Christianson 1
Affiliation  

The year 2017 marks the twentieth anniversary of terpenoid cyclase structural biology: a trio of terpenoid cyclase structures reported together in 1997 were the first to set the foundation for understanding the enzymes largely responsible for the exquisite chemodiversity of more than 80000 terpenoid natural products. Terpenoid cyclases catalyze the most complex chemical reactions in biology, in that more than half of the substrate carbon atoms undergo changes in bonding and hybridization during a single enzyme-catalyzed cyclization reaction. The past two decades have witnessed structural, functional, and computational studies illuminating the modes of substrate activation that initiate the cyclization cascade, the management and manipulation of high-energy carbocation intermediates that propagate the cyclization cascade, and the chemical strategies that terminate the cyclization cascade. The role of the terpenoid cyclase as a template for catalysis is paramount to its function, and protein engineering can be used to reprogram the cyclization cascade to generate alternative and commercially important products. Here, I review key advances in terpenoid cyclase structural and chemical biology, focusing mainly on terpenoid cyclases and related prenyltransferases for which X-ray crystal structures have informed and advanced our understanding of enzyme structure and function.

中文翻译:

萜类环化酶的结构和化学生物学。

2017年是萜类环化酶结构生物学的二十周年纪念:1997年一起报道的三类萜类环化酶结构是第一个为了解主要负责8万多种萜类天然产物的精细化学多样性的酶奠定基础的方法。萜类环化酶催化生物学上最复杂的化学反应,因为在单个酶催化的环化反应过程中,超过一半的底物碳原子会发生键合和杂交的变化。在过去的二十年中,进行了结构,功能和计算研究,这些研究阐明了引发环化级联反应的底物活化模式,传播环化级联反应的高能碳正离子中间体的管理和操作,以及终止环化级联反应的化学策略。萜类环化酶作为催化模板的作用对其功能至关重要,蛋白质工程可用于重新编程环化级联反应,以产生替代的和重要的商业产品。在这里,我回顾了萜类环化酶的结构和化学生物学的主要进展,主要侧重于萜类环化酶和相关的异戊二烯基转移酶,它们的X射线晶体结构为我们提供了信息,并加深了我们对酶结构和功能的理解。
更新日期:2017-08-25
down
wechat
bug