Microchip Circulation Drastically Accelerates Amyloid Aggregation of 1–42 β-amyloid Peptide from Felis catus,ACS Chemical Neuroscience

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Microchip Circulation Drastically Accelerates Amyloid Aggregation of 1–42 β-amyloid Peptide from Felis catus
ACS Chemical Neuroscience ( IF 4.1 ) Pub Date : 2017-08-25 00:00:00 , DOI: 10.1021/acschemneuro.7b00285
Witold Gospodarczyk ₁ , Maciej Kozak _{1,

2}

Affiliation

The amyloid aggregation process of amyloid β_1–42 peptide is responsible for Alzheimer’s disease, affecting millions of elderly people worldwide. Although there has been a great deal of attention directed toward tackling this disease, still no medicine has been found for this fatal disorder. To address this challenge, it is vital to thoroughly understand the molecular mechanism underlying the amyloid peptide aggregation process, as well as seek substances that could hamper this aggregation. In order to shed light on mechanisms leading to amyloidogenesis, we employed a microfluidic system to determine the possible influence of in vivo-like flow in the microchip channel itself on feline Aβ_1–42 peptide amyloidogenesis. We have shown that shear forces occurring during such flow immensely accelerated peptide aggregation. We also tested the inhibitory influence of 3,3′-[1,6-(2,5-dioxahexane)]bis(1-dodecylimidazolium) dichloride gemini surfactant on peptide amyloidogenesis. Our results suggest that this surfactant may inhibit amyloid β_1–42 fibril formation.

中文翻译：

1-42β淀粉样肽的Microchip的流通急剧能够加速淀粉样蛋白聚集家猫

β淀粉样蛋白的淀粉样蛋白聚集过程_1-42肽负责阿尔茨海默氏病，影响了全世界数百万的老年人。尽管已经有很多针对这种疾病的治疗方法，但仍没有发现用于治疗这种致命疾病的药物。为了应对这一挑战，至关重要的是要彻底了解淀粉样蛋白肽聚集过程的分子机制，并寻找可能阻碍这种聚集的物质。为了阐明导致淀粉样蛋白生成的机制，我们采用了微流体系统来确定微芯片通道本身的类体内流对猫_Aβ1–42的可能影响肽淀粉样蛋白生成。我们已经表明，在这种流动过程中发生的剪切力极大地加速了肽的聚集。我们还测试了3,3'-[1,6-（2,5-二氧杂己烷）]双（1-十二烷基咪唑鎓）二氯化物双子表面活性剂对肽淀粉样蛋白生成的抑制作用。我们的结果表明，该表面活性剂可能抑制淀粉样蛋白_β1–42的原纤维形成。

更新日期：2017-08-25

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