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Magnetic particles assisted capture and release of rare circulating tumor cells using wavy-herringbone structured microfluidic devices
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-08-10 00:00:00 , DOI: 10.1039/c7lc00333a Wentao Shi 1, 2, 3, 4 , Shunqiang Wang 2, 3, 4, 5 , Ahmad Maarouf 1, 2, 3, 4 , Christopher G. Uhl 1, 2, 3, 4 , Ran He 2, 3, 4, 5 , Doruk Yunus 2, 3, 4, 5 , Yaling Liu 1, 2, 3, 4, 5
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-08-10 00:00:00 , DOI: 10.1039/c7lc00333a Wentao Shi 1, 2, 3, 4 , Shunqiang Wang 2, 3, 4, 5 , Ahmad Maarouf 1, 2, 3, 4 , Christopher G. Uhl 1, 2, 3, 4 , Ran He 2, 3, 4, 5 , Doruk Yunus 2, 3, 4, 5 , Yaling Liu 1, 2, 3, 4, 5
Affiliation
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A wavy-herringbone (wavy-HB) structured microfluidic device was used to effectively and selectively capture and release circulating tumor cells (CTCs) by using immunoaffinity and magnetic force. This device was designed to create passive turbulence and increase the possibility of tumor cells colliding with the device wall. Under an external magnetic field, magnetic particles (MPs) coated with anti-EpCAM against a tumor cell surface protein (EpCAM) were immobilized over the wavy-HB surface to capture tumor cells. After removing the magnetic field, the captured cells with surplus MPs were released from the device and collected; thus, these cells could be re-cultured for further analysis. Under optimized conditions, the capture efficiency of the tumor cells can be as high as 92% ± 2.8%. Capture experiments were also performed on whole blood samples, and the capture efficiency was in a high range of 81–95%, at different tumor cell concentrations. Such a method can potentially be used for CTC sorting from patient blood samples, CTC concentration monitoring, therapeutic guidance and drug dosage choice, and further study of tumors, such as drug screening and tumor mutations.
中文翻译：
波浪形人字形微流控装置可帮助磁性粒子捕获和释放罕见的循环肿瘤细胞
使用波浪形人字形（wavy-HB）结构的微流体装置，通过免疫亲和力和磁力有效地，选择性地捕获和释放循环肿瘤细胞（CTC）。该设备旨在产生被动湍流，并增加肿瘤细胞与设备壁碰撞的可能性。在外部磁场下，将涂有抗肿瘤细胞表面蛋白（EpCAM）的抗EpCAM的磁性颗粒（MPs）固定在波浪状HB表面上，以捕获肿瘤细胞。去除磁场后，捕获的具有多余MP的细胞会从设备中释放出来并收集。因此，可以重新培养这些细胞以进行进一步分析。在最佳条件下，肿瘤细胞的捕获效率可高达92％±2.8％。还对全血样本进行了捕获实验，在不同的肿瘤细胞浓度下，捕获效率在81％至95％的高范围内。这种方法可潜在地用于从患者血液样本中进行CTC分选，CTC浓度监测，治疗指导和药物剂量选择，以及进一步研究肿瘤，例如药物筛选和肿瘤突变。
更新日期：2017-08-25
中文翻译：
波浪形人字形微流控装置可帮助磁性粒子捕获和释放罕见的循环肿瘤细胞
使用波浪形人字形（wavy-HB）结构的微流体装置，通过免疫亲和力和磁力有效地，选择性地捕获和释放循环肿瘤细胞（CTC）。该设备旨在产生被动湍流，并增加肿瘤细胞与设备壁碰撞的可能性。在外部磁场下，将涂有抗肿瘤细胞表面蛋白（EpCAM）的抗EpCAM的磁性颗粒（MPs）固定在波浪状HB表面上，以捕获肿瘤细胞。去除磁场后，捕获的具有多余MP的细胞会从设备中释放出来并收集。因此，可以重新培养这些细胞以进行进一步分析。在最佳条件下，肿瘤细胞的捕获效率可高达92％±2.8％。还对全血样本进行了捕获实验，在不同的肿瘤细胞浓度下，捕获效率在81％至95％的高范围内。这种方法可潜在地用于从患者血液样本中进行CTC分选，CTC浓度监测，治疗指导和药物剂量选择，以及进一步研究肿瘤，例如药物筛选和肿瘤突变。
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