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Regulatory Mechanism of Mycotoxin Tenuazonic Acid Production in Pyricularia oryzae
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2017-08-24 00:00:00 , DOI: 10.1021/acschembio.7b00353 Choong-Soo Yun 1 , Takayuki Motoyama 1 , Hiroyuki Osada 1
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2017-08-24 00:00:00 , DOI: 10.1021/acschembio.7b00353 Choong-Soo Yun 1 , Takayuki Motoyama 1 , Hiroyuki Osada 1
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Tenuazonic acid (TeA) is a mycotoxin produced by the rice blast fungus Pyricularia oryzae and some plant pathogenic fungi. We previously demonstrated that TeA is biosynthesized in P. oryzae by TeA synthetase 1 (TAS1) and that its production is induced by osmo-sensory MAPK-encoding gene (OSM1) deletion or the addition of 1% DMSO to cultures; however, the regulatory mechanisms of TeA production were unknown. Here, we identify a Zn(II)2-Cys6-type transcription factor in the upstream region of TAS1, which is encoded by TAS2 and regulates TeA production. We also find PoLAE1, which is a homologue of LaeA, a regulator of fungal secondary metabolism. Analysis of PoLAE1 deletion and overexpression strains indicate that PoLAE1 drives TeA production. We also demonstrate that two TeA-inducing signals, 1% DMSO addition and OSM1 deletion, were transmitted through PoLAE1. Our results indicate that TeA production is regulated by two specific regulators, TAS2 and PoLAE1, in P. oryzae.
中文翻译：
稻瘟病菌中霉菌毒素替硝唑酸产生的调控机制
Tenuazonic acid（TeA）是稻瘟病菌稻瘟病菌（Pyricularia oryzae）和某些植物病原真菌产生的霉菌毒素。我们先前证明，TeA是通过TeA合成酶1（TAS1）在稻瘟病菌中生物合成的，并且其产生是由渗透感应MAPK编码基因（OSM1）缺失或向培养物中添加1％DMSO诱导的。然而，TeA生产的调控机制尚不清楚。在这里，我们确定了TAS1上游区域中由TAS2编码的Zn（II）2 -Cys 6型转录因子并规范TeA的生产。我们还发现PoLAE1，它是LaeA（真菌次级代谢的调节剂）的同源物。分析PoLAE1缺失和过表达菌株表明PoLAE1推动茶叶生产。我们还证明了通过PoLAE1传输了两个诱导TeA的信号，即1％DMSO添加和OSM1缺失。我们的研究结果表明，茶叶生产是由两个特定的监管机构，TAS2和PoLAE1，在调控P.菌。
更新日期：2017-08-25
中文翻译：
稻瘟病菌中霉菌毒素替硝唑酸产生的调控机制
Tenuazonic acid（TeA）是稻瘟病菌稻瘟病菌（Pyricularia oryzae）和某些植物病原真菌产生的霉菌毒素。我们先前证明，TeA是通过TeA合成酶1（TAS1）在稻瘟病菌中生物合成的，并且其产生是由渗透感应MAPK编码基因（OSM1）缺失或向培养物中添加1％DMSO诱导的。然而，TeA生产的调控机制尚不清楚。在这里，我们确定了TAS1上游区域中由TAS2编码的Zn（II）2 -Cys 6型转录因子并规范TeA的生产。我们还发现PoLAE1，它是LaeA（真菌次级代谢的调节剂）的同源物。分析PoLAE1缺失和过表达菌株表明PoLAE1推动茶叶生产。我们还证明了通过PoLAE1传输了两个诱导TeA的信号，即1％DMSO添加和OSM1缺失。我们的研究结果表明，茶叶生产是由两个特定的监管机构，TAS2和PoLAE1，在调控P.菌。
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