Menadione, as the crucial component of vitamin Ks, possessed significant nutritional and clinical values. However, there was still lack of favourable quantification strategies for it to date. For improvement, a novel cysteamine derivatization based UPLC–MS/MS method was presented in this work. The derivatizating reaction was proved non-toxic, easy-handling and high-efficient, which realized the MS detection of menadione under positive mode. Benefitting from the excellent sensitivity of the derivatizating product as well as the introduction of the stable isotope dilution technique, the quantification could be achieved in the range of 0.05–50.0 ng/mL for plasma and urine matrixes with satisfied accuracy and precision. After analysis of the samples from healthy volunteers after oral administration of menadione sodium bisulfite tablets, the urinary free menadione was quantified for the very first time. We believe the progress in this work could largely promote the exploration of the metabolic mechanism of vitamin K in vivo.

甲萘醌作为维生素Ks的关键成分，具有重要的营养和临床价值。但是，迄今为止，仍缺乏有利的量化策略。为了改进，本文提出了一种基于UPLC-MS / MS的新型半胱胺衍生化方法。衍生化反应被证明是无毒，易处理，高效的，实现了MS在正模式下对甲萘醌的检测。得益于衍生化产品的出色灵敏度以及稳定同位素稀释技术的引入，血浆和尿液基质的定量可在0.05-50.0 ng / mL范围内实现，并具有满意的准确度和精密度。在对口服甲萘醌亚硫酸氢钠钠片的健康志愿者的样品进行分析后，首次对尿中游离甲萘醌进行了定量。我们相信这项工作的进展将在很大程度上促进对维生素K代谢机制的探索。体内。