In this paper, a trimetallic [email protected]@Pt nanocomposites platform on -COOH terminated reduced graphene oxide (COOH-rGO) was newly developed for sensing carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) biomarkers. Trimetallic electro-catalytic surfaces were prepared by the electrodeposition of noble metals ([email protected]@Pt) nanoparticles on COOH- rGO. After EDC/NHS treatment, the anti-CEA and anti-PSA were immobilized separately on two different platforms. Under optimized conditions, the platforms were analyzed by cyclic voltammetry and differential pulse voltammetry (DPV). The platform shows good electro catalytic activity, high sensitivity, and acceptable stability for sensing CEA and PSA biomarkers. For CEA, we obtained sensitivity of 0.099 ± 0.007 µA ng⁻¹ ml, wide linear range from 12 pg ml⁻¹ to 85 ng ml⁻¹ and a limit of detection (LOD) of 8 pg ml⁻¹, while for PSA sensitivity is 0.267 ± 0.02 µA ng ⁻¹ ml, wide linear range from 3 pg ml⁻¹ to 60 ng ml⁻¹ and LOD of 2 pg ml⁻¹. The validation of the platform was observed through standard addition method. Thus, the sensing platform could be used for the point of care detection of CEA and PSA.

在本文中，新开发了一种在-COOH末端还原的氧化石墨烯（COOH-rGO）上的三金属[电子邮件保护] @Pt纳米复合材料平台，用于检测癌胚抗原（CEA）和前列腺特异性抗原（PSA）生物标志物。通过将贵金属（[电子邮件保护] @Pt）纳米粒子电沉积在COOH-rGO上，制备了三金属电催化表面。在EDC / NHS处理后，将抗CEA和抗PSA分别固定在两个不同的平台上。在优化条件下，通过循环伏安法和差分脉冲伏安法（DPV）对平台进行了分析。该平台在感应CEA和PSA生物标志物方面显示出良好的电催化活性，高灵敏度和可接受的稳定性。对于CEA，我们获得了0.099±0.007 µA ng ^-1的灵敏度 ml，线性范围从12 pg ml ^-1到85 ng ml ^-1，检测极限（LOD）为8 pg ml ^-1，而PSA灵敏度为0.267±0.02 µA ng ^-1 ml，线性范围从3 pg ml ^-1至60 ng ml ^-1的LOD为2 pg ml ^-1。通过标准添加方法对平台进行了验证。因此，该感测平台可用于CEA和PSA的护理点检测。