In the present investigation, extraction and preconcentration of methamphetamine in human urine samples was carried out using a novel bio-dispersive liquid liquid microextraction (Bio-DLLME) technique coupled with magnetic solid phase extraction (MSPE). Bio-DLLME is a kind of microextraction technique based nano-materials which have potential capabilities in many application fields. Bio-DLLME is based on the use of a binary part system consisting of methanol and nano rhaminolipid biosurfactant. Use of this binary mixture is ecologically accepted due to their specificity, biocompatibility and biodegradable nature. The potential of nano rhaminolipid biosurfactant as a biological agent in the extraction of organic compounds has been investigated in recent years. They are able to partition at the oil/water interfaces and reduce the interfacial tension in order to increase solubility of hydrocarbons. The properties of the prepared Fe₃O₄@PPy magnetic nanoparticles were characterized using Fourier transform infrared spectroscopy and X-ray diffraction methods The influences of the experimental parameters on the quantitative recovery of analyte were investigated. Under optimized conditions, the enrichment factor was 310, the calibration graph was linear in the methamphetamine concentration range from 1 to 60 μg L⁻¹, with a correlation coefficient of 0.9998. The relative standard deviations for six replicate measurements was 5.2%.

在本研究中，使用新型生物分散液微萃取（Bio-DLLME）技术与磁性固相萃取（MSPE）结合进行了人尿液中甲基苯丙胺的提取和预浓缩。Bio-DLLME是一种基于微萃取技术的纳米材料，在许多应用领域中具有潜在的功能。Bio-DLLME是基于使用由甲醇和纳米Rhaminolipidid生物表面活性剂组成的二元系统的。这种二元混合物由于其特异性，生物相容性和可生物降解的性质而在生态学上被接受。近年来，已经研究了纳米级鼻氨基脂质生物表面活性剂作为生物试剂提取有机化合物的潜力。它们能够在油/水界面处分配并降低界面张力，以增加碳氢化合物的溶解度。制备铁的性质利用傅里叶变换红外光谱和X射线衍射方法对₃ O ₄ @PPy磁性纳米粒子进行了表征。研究了实验参数对分析物定量回收的影响。在优化条件下，富集因子为310，在甲基苯丙胺浓度范围为1至60μgL ^-1时，校准图呈线性，相关系数为0.9998。六次重复测量的相对标准偏差为5.2％。