Development of a Recombinant Multifunctional Biomacromolecule for Targeted Gene Transfer to Prostate Cancer Cells,Biomacromolecules

当前位置： X-MOL 学术 › Biomacromolecules › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Development of a Recombinant Multifunctional Biomacromolecule for Targeted Gene Transfer to Prostate Cancer Cells
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-08-24 00:00:00 , DOI: 10.1021/acs.biomac.7b00739
Arash Hatefi _{1,

2} , Zahra Karjoo ₁ , Alireza Nomani ₁

Affiliation

The objective of this study was to genetically engineer a fully functional single chain fusion peptide composed of motifs from diverse biological and synthetic origins that can perform multiple tasks including DNA condensation, cell targeting, cell transfection, particle shielding from immune system and effective gene transfer to prostate tumors. To achieve the objective, a single chain biomacromolecule (vector) consisted of four repeatative units of histone H2A peptide, fusogenic peptide GALA, short elastin-like peptide, and PC-3 cell targeting peptide was designed. To examine the functionality of each motif in the vector sequence, it was characterized in terms of size and zeta potential by Zetasizer, PC-3 cell targeting and transfection by flowcytometry, IgG induction by immunogenicity assay, and PC-3 tumor transfection by quantitative live animal imaging. Overall, the results of this study showed the possibility of using genetic engineering techniques to program various functionalities into one single chain vector and create a multifunctional nonimmunogenic biomacromolecule for targeted gene transfer to prostate cancer cells. This proof-of-concept study is a significant step forward toward creating a library of vectors for targeted gene transfer to any cancer cell type at both in vitro and in vivo levels.

中文翻译：

靶向基因转移到前列腺癌细胞的重组多功能生物大分子的发展。

这项研究的目的是对全功能的单链融合肽进行基因工程改造，该肽由来自不同生物学和合成来源的基序组成，可以执行多种任务，包括DNA缩合，细胞靶向，细胞转染，免疫系统的颗粒屏蔽以及有效的基因转移。前列腺肿瘤。为了实现该目的，设计了由组蛋白H2A肽，融合肽GALA，短弹性蛋白样肽和PC-3细胞靶向肽的四个重复单元组成的单链生物大分子（载体）。为了检查载体序列中每个基序的功能，通过Zetasizer对其大小和Zeta电位，通过流式细胞术对PC-3细胞进行靶向和转染，通过免疫原性测定进行IgG诱导，通过定量活体动物成像检测PC-3肿瘤。总的来说，这项研究的结果表明，可以使用基因工程技术将各种功能编程到一个单链载体中，并创建一种多功能的非免疫原性生物大分子，用于靶向基因转移至前列腺癌细胞。这项概念验证研究是朝着建立用于在体外和体内水平将靶向基因转移至任何癌细胞类型的载体库迈出的重要一步。

更新日期：2017-08-24

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11