AbstractThe authors describe a method for the detection of DNA by using immobilized molecular beacons (MBs) on the surface of silicon, with a view on possible application in biosensing. MB hybridization and protein recognition were interrogated on silicon-on-insulator (SOI) surfaces by using fluorescently tagged probes. In order to better understand the conformational changes that occur to MBs upon hybridization, the process was studied by using dual polarization interferometry (DPI). A model system was developed that matches thickness, mass, and density parameters. The results experimentally demonstrate that hybridization promotes the displacement of a protein away from the surface. This finding may be further exploited in techniques such as photonic sensors, thereby paving the way to the design of more sensitive biosensors based on the use of MBs. Graphical abstractSchematic of a new DNA/RNA detection scheme by using immobilized molecular beacons (MBs) on silicon, with a view on possible application in biosensing. A study was performed on the conformational changes that occur to MBs upon hybridization by Dual Polarization Interferometry (DPI).

中文翻译：

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通过硫醇-烯偶联共价连接生物素化分子信标。杂交和链霉亲和素结合后构象变化的研究

摘要作者描述了一种通过在硅表面上使用固定化分子信标 (MB) 来检测 DNA 的方法，以期在生物传感中的可能应用。MB 杂交和蛋白质识别通过使用荧光标记的探针在绝缘体上硅 (SOI) 表面进行询问。为了更好地了解杂交后 MB 发生的构象变化，通过使用双偏振干涉测量法 (DPI) 研究了该过程。开发了匹配厚度、质量和密度参数的模型系统。实验结果表明，杂交促进了蛋白质从表面的置换。这一发现可能会在光子传感器等技术中得到进一步利用，从而为基于 MB 的使用设计更灵敏的生物传感器铺平了道路。图形摘要通过在硅上使用固定化分子信标 (MB) 的新 DNA/RNA 检测方案的示意图，并考虑在生物传感中的可能应用。通过双偏振干涉测量法 (DPI) 对 MB 在杂交时发生的构象变化进行了研究。