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Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
Molecular Cell ( IF 14.5 ) Pub Date : 2017-07-20 , DOI: 10.1016/j.molcel.2017.06.029 Yong Xue 1 , Suman K Pradhan 1 , Fei Sun 1 , Constantinos Chronis 1 , Nancy Tran 1 , Trent Su 1 , Christopher Van 2 , Ajay Vashisht 1 , James Wohlschlegel 1 , Craig L Peterson 2 , H T Marc Timmers 3 , Siavash K Kurdistani 1 , Michael F Carey 1
Molecular Cell ( IF 14.5 ) Pub Date : 2017-07-20 , DOI: 10.1016/j.molcel.2017.06.029 Yong Xue 1 , Suman K Pradhan 1 , Fei Sun 1 , Constantinos Chronis 1 , Nancy Tran 1 , Trent Su 1 , Christopher Van 2 , Ajay Vashisht 1 , James Wohlschlegel 1 , Craig L Peterson 2 , H T Marc Timmers 3 , Siavash K Kurdistani 1 , Michael F Carey 1
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Pervasive transcription initiates from cryptic promoters and is observed in eukaryotes ranging from yeast to mammals. The Set2-Rpd3 regulatory system prevents cryptic promoter function within expressed genes. However, conserved systems that control pervasive transcription within intergenic regions have not been well established. Here we show that Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 co-localize on chromatin and coordinately suppress pervasive transcription in S. cerevisiae and murine embryonic stem cells (mESCs). In yeast, all three proteins bind subtelomeric heterochromatin through a Sir3-stimulated mechanism and to euchromatin via a TBP-stimulated mechanism. In mESCs, the proteins bind to active and poised TBP-bound promoters along with promoters of polycomb-silenced genes apparently lacking TBP. Depletion of Mot1, Ino80C, or NC2 by anchor away in yeast or RNAi in mESCs leads to near-identical transcriptome phenotypes, with new subtelomeric transcription in yeast, and greatly increased pervasive transcription in both yeast and mESCs.
中文翻译：
Mot1，Ino80C和NC2协同调节酵母和哺乳动物的普遍转录。
普遍的转录从隐秘的启动子开始，并在从酵母到哺乳动物的真核生物中观察到。Set2-Rpd3调节系统可防止表达基因内的隐秘启动子功能。然而，尚未很好地建立控制基因间区域内普遍转录的保守系统。在这里，我们显示Mot1，Ino80染色质重塑复合物（Ino80C）和NC2在染色质上共定位并协调抑制酿酒酵母和鼠类胚胎干细胞（mESCs）中的普遍转录。在酵母中，所有三种蛋白质均通过Sir3刺激的机制结合亚端粒异染色质，并通过TBP刺激的机制与常染色质结合。在mESC中，蛋白质与活跃且平衡的TBP结合的启动子以及显然缺乏TBP的多梳沉默基因的启动子结合。Mot1，Ino80C，
更新日期：2017-07-20
中文翻译：
Mot1，Ino80C和NC2协同调节酵母和哺乳动物的普遍转录。
普遍的转录从隐秘的启动子开始，并在从酵母到哺乳动物的真核生物中观察到。Set2-Rpd3调节系统可防止表达基因内的隐秘启动子功能。然而，尚未很好地建立控制基因间区域内普遍转录的保守系统。在这里，我们显示Mot1，Ino80染色质重塑复合物（Ino80C）和NC2在染色质上共定位并协调抑制酿酒酵母和鼠类胚胎干细胞（mESCs）中的普遍转录。在酵母中，所有三种蛋白质均通过Sir3刺激的机制结合亚端粒异染色质，并通过TBP刺激的机制与常染色质结合。在mESC中，蛋白质与活跃且平衡的TBP结合的启动子以及显然缺乏TBP的多梳沉默基因的启动子结合。Mot1，Ino80C，
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