Trends in Cell Biology ( IF 13.0 ) Pub Date : 2017-06-12 , DOI: 10.1016/j.tcb.2017.05.003 Maximilian Brackmann , Sergey Nazarov , Jing Wang , Marek Basler
Using physical force to translocate macromolecules across a membrane has the advantage of being a universal solution independent of the properties of the target membrane. However, physically punching a stiff membrane is not a trivial task and three things are necessary for success: a sharp tip, a source of energy, and the ability to strongly bind to the target. In this review we describe the basic mechanism of membrane puncturing by contractile nanomachines with a focus on the T4 phage, R-type pyocin, and the bacterial Type VI secretion system (T6SS) based on recent studies of the structures and dynamics of their assembly.
中文翻译:
用力冲孔:可收缩纳米机的力学
使用物理力使大分子跨膜移位具有以下优点:它是一种通用解决方案,与目标膜的特性无关。但是,对坚硬的膜片进行物理穿孔并不是一件容易的事,成功需要三件事:尖的尖端,能量的来源以及与目标牢固结合的能力。在这篇综述中,我们基于对结构和组装动力学的最新研究,描述了收缩性纳米机器对膜的穿刺的基本机制,重点是T4噬菌体,R型脓菌素和细菌VI型分泌系统(T6SS)。