Annual Review of Cell and Developmental Biology ( IF 11.4 ) Pub Date : 2016-10-06 00:00:00 , DOI: 10.1146/annurev-cellbio-111315-125016 Charles Barlowe 1 , Ari Helenius 2
Transport of newly synthesized proteins from the endoplasmic reticulum (ER) to the Golgi complex is highly selective. As a general rule, such transport is limited to soluble and membrane-associated secretory proteins that have reached properly folded and assembled conformations. To secure the efficiency, fidelity, and control of this crucial transport step, cells use a combination of mechanisms. The mechanisms are based on selective retention of proteins in the ER to prevent uptake into transport vesicles, on selective capture of proteins in COPII carrier vesicles, on inclusion of proteins in these vesicles by default as part of fluid and membrane bulk flow, and on selective retrieval of proteins from post-ER compartments by retrograde vesicle transport.
中文翻译:
早期分泌途径中的货物捕获和散装流动
新合成的蛋白质从内质网(ER)到高尔基体的转运是高度选择性的。通常,这种运输仅限于已达到适当折叠和组装构象的可溶性和膜相关分泌蛋白。为了确保此关键运输步骤的效率,保真度和控制力,细胞使用了多种机制的组合。这些机制基于选择性保留在ER中的蛋白质以防止摄取到运输小泡中,选择性捕获COPII载体小泡中的蛋白质,默认情况下将这些小泡中的蛋白质作为流体和膜体积流量的一部分以及选择性通过逆行囊泡运输从后ER隔室中检索蛋白质。