A dual-responsive, cell capture and release surface was prepared through the incorporation of phenylboronic acid (PBA) groups into an oxime-based polyethylene glycol (PEG) hydrogel. Owing to its PEG-like properties, the unfunctionalized hydrogel was nonfouling. The use of highly efficient oxime chemistry allows the incorporation of commercially available 3,5-diformylphenyl boronic acid into the hydrogel matrix. Thus, the surface properties of the hydrogel were modified to enable reversible cell capture and release. Boronic ester formation between PBA groups and cell surface carbohydrates enabled efficient cell capture at pH 6.8. An increase to pH 7.8 resulted in cell detachment. This capture-and-release procedure was performed on MCF-7 human breast cancer cells, NIH-3T3 fibroblast cells, and primary human umbilical vein endothelial cells (HUVECs) and could be cycled with negligible loss in activity. The facile preparation of PBA-functionalized surfaces presented here has applications in biomedical fields such as cell diagnostics and cell culture.

中文翻译：

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动态共价水凝胶用于触发细胞捕获和释放

通过将苯基硼酸（PBA）基团掺入基于肟的聚乙二醇（PEG）水凝胶中，制备了双重响应的细胞捕获和释放表面。由于其类似PEG的特性，未官能化的水凝胶不会结垢。高效肟化学的使用允许将可商购的3，5-二甲酰基苯基硼酸掺入到水凝胶基质中。因此，水凝胶的表面性质被修饰以使得可逆的细胞捕获和释放成为可能。PBA基团与细胞表面碳水化合物之间形成硼酸酯，可在pH 6.8时有效捕获细胞。pH值7.8升高导致细胞脱离。此捕获和释放过程是在MCF-7人乳腺癌细胞，NIH-3T3成纤维细胞，和原代人脐静脉内皮细胞（HUVEC），并且可以以很少的活性损失进行循环。本文介绍的PBA功能化表面的简便制备方法已在生物医学领域中应用，例如细胞诊断和细胞培养。