Impact of the biomolecular corona on the structure of PEGylated liposomes†,Biomaterials Science

当前位置： X-MOL 学术 › Biomater. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Impact of the biomolecular corona on the structure of PEGylated liposomes†
Biomaterials Science ( IF 5.8 ) Pub Date : 2017-06-19 00:00:00 , DOI: 10.1039/c7bm00387k
Luca Digiacomo _{1,

2,

3,

4,

5} , Daniela Pozzi _{1,

2,

3,

4} , Heinz Amenitsch _{6,

7,

8,

9} , Giulio Caracciolo _{1,

2,

3,

4}

Affiliation

Driven by the promises of gene therapy, PEGylated cationic liposomes (CLs) have been investigated for decades, but their use in the clinical setting is far from established. Such a dichotomy is due to several factors that have been ignored over the last two decades. The hardest challenge seems to occur when PEGylated CLs come into contact with a physiological environment (e.g. the blood). Recent evidence has demonstrated that PEGylation does not completely prevent protein binding (as believed so far), but a biomolecular shell, termed “biomolecular corona” (BC), covers the liposome surface. Here we show that the formation of a BC not only affects the surface properties of PEGylated CLs, but also, and significantly, their bilayer structure thus impairing their ability to safely deliver their cargo to the target site. Therefore, a mechanistic understanding of the structures emerging from liposome–protein interactions may represent a truly new paradigm for the clinical translation of PEGylated CLs.

中文翻译：

生物分子电晕对聚乙二醇化脂质体结构的影响†

受基因治疗前景的驱动，聚乙二醇化阳离子脂质体（CLs）已有数十年的研究历史，但在临床上的应用还远未建立。这种二分法是由于过去二十年中被忽略的几个因素造成的。当PEG化的CL与生理环境接触时，最困难的挑战似乎发生了（例如血液）。最近的证据表明，聚乙二醇化不能完全阻止蛋白质的结合（到目前为止，人们一直认为），但是被称为“生物分子电晕”（BC）的生物分子壳覆盖了脂质体的表面。在这里，我们表明BC的形成不仅影响PEG化CL的表面特性，而且显着影响其双层结构，从而损害其将货物安全运送到目标位置的能力。因此，对脂质体-蛋白质相互作用中出现的结构的机械理解可能代表了PEG化CL的临床翻译的真正新范例。

更新日期：2017-06-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11