ε-Polylysine-based thermo-responsive adsorbents for immunoglobulin adsorption–desorption under mild conditions†,Biomaterials Science

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ε-Polylysine-based thermo-responsive adsorbents for immunoglobulin adsorption–desorption under mild conditions†
Biomaterials Science ( IF 5.8 ) Pub Date : 2017-06-08 00:00:00 , DOI: 10.1039/c7bm00390k
Masashi Maruyama _{1,

2,

3,

4,

5} , Keisuke Shibuya _{1,

2,

3,

4,

5}

Affiliation

Thermo-responsive adsorbents for immunoglobulin G (IgG) employing ε-polylysine (EPL) as a polymer backbone were developed. The introduction of mercaptoethylpyridine (MEP) as an IgG-binding ligand and hydrophobization of side chains afforded thermo-responsive IgG adsorbents, whose thermo-responsive IgG desorption ratio was up to 88% (EPL/MEP derivative 3m). The changes in surface densities of active MEP groups, which are caused by thermal conformational changes of the adsorbents, play key roles for IgG desorption. Although a trade-off of IgG adsorption capacity and IgG desorption ratio was observed, the present study offers a novel molecular design for thermo-responsive adsorbents with high synthetic accessibility and potentially low toxicity.

中文翻译：

基于ε-聚赖氨酸的热响应型吸附剂，在温和条件下用于免疫球蛋白的吸附-解吸†

开发了以ε-聚赖氨酸（EPL）为聚合物骨架的免疫球蛋白G（IgG）的热敏吸附剂。巯基乙基吡啶（MEP）作为IgG结合配体的引入和侧链的疏水化提供了热响应性IgG吸附剂，其热响应性IgG解吸率高达88％（EPL / MEP衍生物3m）。活性MEP基团的表面密度变化是由吸附剂的热构象变化引起的，在IgG解吸中起关键作用。尽管观察到了IgG吸附能力和IgG解吸比之间的折衷，但本研究为热响应性吸附剂提供了一种新颖的分子设计，该吸附剂具有较高的合成可达性和潜在的低毒性。

更新日期：2017-06-08

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