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Color and alcohol removal for the simultaneous detection of amino acids and sugars in wine by two-dimensional ion chromatography
Journal of Chromatography B ( IF 2.8 ) Pub Date : 2017-08-18 , DOI: 10.1016/j.jchromb.2017.08.017
Yun Fa , Yinghui Liu , Aihua Xu , Yuexue Yu , Fangfang Li , Huizhou Liu

An effective pretreatment method for wine color removal by a PS-DVB SPE cartridge and online alcohol elimination by valve switching was presented. The optimum parameters for color removal were investigated: 40-μm and 100 Å poly (styrene)-divinylbenzene (PS-DVB) (0.4 g) was selected as the color removal material and 5 mL of ethanol (10%) as the elution solvent for sample pretreatment under given condition. Moreover, an accurate and automated two-dimensional ion chromatography method for the simultaneous detection of amino acids and sugars was achieved with two valves after injection without alcohol interference. The method had a mean correlation coefficient of >0.99 and a repeatability of 0.92%–4.30% for eight replicates. The mean recovery of six red wine samples were 97.6%, 96.6%, 96.1%, 95.9%, 97.3% and 96.4% respectively. And this method successfully analyzed the amino acid and sugar contents of six wine samples of different origins.



中文翻译:

去除颜色和酒精,通过二维离子色谱法同时检测葡萄酒中的氨基酸和糖

提出了一种有效的预处理方法,用于通过PS-DVB SPE滤芯去除酒色并通过阀切换在线消除酒精。研究了用于除色的最佳参数:选择40-μm和100Å聚(苯乙烯)-二乙烯基苯(PS-DVB)(0.4 g)作为除色材料,并使用5 mL乙醇(10%)作为洗脱溶剂在给定条件下进行样品预处理。此外,在注射后用两个阀门实现了准确自动的二维离子色谱法,可同时检测氨基酸和糖类,而无酒精干扰。该方法的平均相关系数> 0.99,八次重复的重复性为0.92%–4.30%。六个红酒样品的平均回收率分别为97.6%,96.6%,96.1%,95.9%,97.3%和96.4%。

更新日期:2017-08-18
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