Metabolic profiling provides an unbiased view of the physiological status of an organism as a “function” of the metabolic composition of a measured sample. Here, we propose a simple LC-MS based workflow for metabolic profiling of volume-restricted samples, namely individual 20 μm-thick histological sections of a mouse kidney. The main idea of this workflow is to re-use the material after an RPLC-MS run, namely using the volume remaining in the vial after injection, and then introducing a phase changing step to enable HILIC-MS analysis. To test the applicability of the workflow and its ability to extract valuable biological information, we applied it to an animal model of polycystic kidney disease (PKD).

中文翻译：

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体积受限组织样品的跨平台代谢组学工作流程：在多囊肾疾病动物模型中的应用

代谢谱分析提供了生物体生理状态作为被测样品代谢成分的“功能”的无偏见。在这里，我们提出了一个基于LC-MS的简单工作流程，用于体积受限样品的代谢谱分析，即小鼠肾脏的20μm厚的组织切片。该工作流程的主要思想是在RPLC-MS运行后重新使用材料，即使用注射后小瓶中剩余的体积，然后引入相变步骤以进行HILIC-MS分析。为了测试工作流程的适用性及其提取有价值的生物学信息的能力，我们将其应用于多囊肾疾病（PKD）的动物模型。