Alantolactone and isoalantolactone isolated from many species of plants are a pair of positional isomers of CC bond. Previously, alantolactone and isoalantolactone have been proved to be good lead compounds for future anticancer agent development. Similarity of their molecular structures increases the separation difficulty for these two isomers on a conventional C₁₈ column. Silver perchlorate (AgClO₄) as mobile phase additives with RP-HPLC for improving the separation was developed for rapid determination of the positional isomers in Inula racemosa Hook.f. The effects of the concentration of silver perchlorate on the separation of the analytes were investigated. The composition of acetonitrile and water containing 5.0% silver perchlorate in a 65:35 (v/v) ratio was used as mobile phase, in which they were well separated within a short period of time on the C₁₈ column. The method was successfully applied to determine them in an extract of Inula racemosa Hook.f. root. Silver perchlorate in mobile phase can efficiently improve the separation of the positional isomers and could be applied to rapidly determinate their content in this plant.

从许多植物物种中分离出的内酯和异戊内酯是一对C C键的位置异构体。以前，已证明丙内酯和异丙内酯是未来抗癌药开发的良好先导化合物。它们分子结构的相似性增加了常规C ₁₈色谱柱上这两种异构体的分离难度。高氯酸银（AgClO ₄），与RP-HPLC流动相添加剂用于改善分离为快速测定位置异构体的开发总状土木香勾子 研究了高氯酸银浓度对分析物分离的影响。乙腈和水的组成为5.0：35（v / v）的5.0％高氯酸银构成的乙腈和水的组合物用作流动相，在短时间内在C ₁₈色谱柱上将它们充分分离。该方法已成功地用于测定油菜（Inula racemosa Hook.f）提取物中的含量。根。流动相中的高氯酸银可有效改善位置异构体的分离，并可用于快速确定其在植物中的含量。