Traceless Cleavage of Protein–Biotin Conjugates under Biologically Compatible Conditions,ChemBioChem

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Traceless Cleavage of Protein–Biotin Conjugates under Biologically Compatible Conditions
ChemBioChem ( IF 2.6 ) Pub Date : 2017-08-16 06:51:48 , DOI: 10.1002/cbic.201700432
Joseph Cowell ₁ , Matthew Buck ₂ , Ali H. Essa _{1,

3} , Rebecca Clarke ₁ , Waldemar Vollmer ₄ , Daniela Vollmer ₄ , Catharien M. Hilkens ₂ , John D. Isaacs ₂ , Michael J. Hall ₁ , Joe Gray ₄

Affiliation

The back cover picture shows RevAmine biotin tag-modified (apo) cytochrome c (bovine) with two accessible lysine residues on the leftmost loop depicted as “ball and stick”. The upper-left lysine is in neutral pH conditions (green background) and therefore still has the RevAmine tag attached, whereas the lower-left lysine has been exposed to bicarbonate buffer, pH 8 (blue background with ions depicted) and as a consequence has tracelessly regenerated the residue along with free biotin and other by-products. More information can be found in the communication by M. J. Hall, J. Gray et al. (DOI: 10.1002/cbic.201700214).

中文翻译：

生物相容性条件下蛋白质-生物素结合物的无痕裂解。

封底图片显示了RevAmine生物素标记修饰的（apo）细胞色素 c（牛），在最左边的环上有两个可接近的赖氨酸残基，描绘为“球和棍棒”。左上方的赖氨酸处于中性pH条件（绿色背景），因此仍附有RevAmine标签，而左下方的赖氨酸已暴露于pH 8的碳酸氢盐缓冲液（蓝色背景，带有离子），因此具有残留物与游离生物素和其他副产物一起无痕地再生。M. J. Hall，J。Gray等人在来文中可以找到更多信息。（DOI：10.1002 / cbic.201700214）。

更新日期：2017-08-16

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