Biochemical Characterization of the Lysine Acetylation of Tyrosyl-tRNA Synthetase in Escherichia coli,ChemBioChem

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Biochemical Characterization of the Lysine Acetylation of Tyrosyl-tRNA Synthetase in Escherichia coli
ChemBioChem ( IF 2.6 ) Pub Date : 2017-08-15 04:30:26 , DOI: 10.1002/cbic.201700343
Sumana Venkat ₁ , Caroline Gregory ₂ , Qinglei Gan ₁ , Chenguang Fan ₁

Affiliation

Reversible lysine acetylation: Site-specifically acetylated Escherichia coli tyrosyl-tRNA synthetase variants were generated by a genetic code expansion strategy. The acetylation of specific lysine residues abolished the aminoacylation activity. The lysine residues could be chemically acetylated by acetyl-phosphate (AcP) and deacetylated by CobB deacetylase in vitro.

中文翻译：

大肠杆菌中酪氨酰-tRNA合成酶的赖氨酸乙酰化的生化特征

可逆的赖氨酸乙酰化：通过遗传密码扩展策略产生位点特异性乙酰化的大肠杆菌酪氨酰-tRNA合成酶变体。特定赖氨酸残基的乙酰化消除了氨基酰化活性。赖氨酸残基可以在体外通过乙酰磷酸（AcP）化学乙酰化，并通过CobB脱乙酰酶脱乙酰。

更新日期：2017-08-15

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