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Immunosensor Employing Stable, Solid 1-Amino-2-naphthyl Phosphate and Ammonia-Borane toward Ultrasensitive and Simple Point-of-Care Testing
ACS Sensors ( IF 8.2 ) Pub Date : 2017-08-14 00:00:00 , DOI: 10.1021/acssensors.7b00407 Jeongwook Seo 1 , Hyeri Ha 1 , Seonhwa Park 1 , Al-Monsur Jiaul Haque 1 , Sinyoung Kim 2 , Jung Min Joo 1 , Haesik Yang 1
ACS Sensors ( IF 8.2 ) Pub Date : 2017-08-14 00:00:00 , DOI: 10.1021/acssensors.7b00407 Jeongwook Seo 1 , Hyeri Ha 1 , Seonhwa Park 1 , Al-Monsur Jiaul Haque 1 , Sinyoung Kim 2 , Jung Min Joo 1 , Haesik Yang 1
Affiliation
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Biosensors for ultrasensitive point-of-care testing require dried reagents with long-term stability and a high signal-to-background ratio. Although ortho-substituted diaromatic dihydroxy and aminohydroxy compounds undergo fast redox reactions, they are not used as electrochemical signaling species because they are readily oxidized and polymerized by dissolved oxygen. In this report, stable, solid 1-amino-2-naphthyl phosphate (1A2N-P) and ammonia-borane (H3N-BH3) are respectively employed as a substrate for alkaline phosphatase (ALP) and a reductant for electrochemical-chemical (EC) redox cycling. ALP converts 1A2N-P to 1-amino-2-naphthol (1A2N), which is then employed in EC redox cycling using H3N-BH3. The oxidation and polymerization of 1A2N by dissolved oxygen is significantly prevented in the presence of H3N-BH3. The electrochemical measurement is performed without modification of indium–tin oxide (ITO) electrodes with electrocatalytic materials. For comparison, nine aromatic dihydroxy and aminohydroxy compounds, including 1A2N, are evaluated to achieve fast EC redox cycling, and four strong reductants, including H3N-BH3, are evaluated to achieve a low background level. The combination of 1A2N and H3N-BH3 allows the achievement of a very high signal-to-background ratio. When the newly developed combination is applied to the detection of creatine kinase-MB (CK-MB), the detection limit for CK-MB is ∼80 fg/mL, indicating that the combination allows ultrasensitive detection. The concentrations of CK-MB in clinical serum samples, determined using the developed system, are in good agreement with the concentrations obtained using a commercial instrument. Thus, the use of stable, solid 1A2N-P and H3N-BH3 along with bare ITO electrodes is highly promising for ultrasensitive and simple point-of-care testing.
中文翻译：
免疫传感器采用稳定的固态1-氨基-2-萘甲酸磷酸盐和氨硼烷进行超灵敏和简单的即时检测
用于超灵敏即时检验的生物传感器需要干燥的试剂，这些试剂必须具有长期的稳定性和很高的信噪比。尽管邻位取代的二芳族二羟基和氨基羟基化合物会经历快速的氧化还原反应，但由于它们易于被溶解的氧氧化和聚合，因此不能用作电化学信号物质。在此报告中，稳定的固态1-氨基-2-萘基磷酸（1A2N-P）和氨硼烷（H 3 N-BH 3）分别用作碱性磷酸酶（ALP）的底物和电化学还原的还原剂。化学（EC）氧化还原循环。ALP将1A2N-P转化为1-氨基-2-萘酚（1A2N），然后使用H 3 N-BH 3将其用于EC氧化还原循环中。在H 3 N-BH 3的存在下，可明显防止1A2N被溶解的氧氧化和聚合。进行电化学测量时，无需用电催化材料修饰氧化铟锡（ITO）电极。为了进行比较，评估了包括1A2N在内的9种芳香族二羟基和氨基羟基化合物以实现快速的EC氧化还原循环，并且评估了包括H 3 N-BH 3在内的4种强还原剂以实现较低的背景水平。1A2N和H 3 N-BH 3的组合可以实现很高的信噪比。当新开发的组合用于肌酸激酶-MB（CK-MB）的检测时，CK-MB的检出限为〜80 fg / mL，表明该组合允许超灵敏的检测。使用开发的系统测定的临床血清样品中的CK-MB浓度与使用商业仪器获得的浓度高度吻合。因此，将稳定，固态的1A2N-P和H 3 N-BH 3与裸露的ITO电极一起使用对于超灵敏和简单的即时检测非常有希望。
更新日期：2017-08-14
中文翻译：
免疫传感器采用稳定的固态1-氨基-2-萘甲酸磷酸盐和氨硼烷进行超灵敏和简单的即时检测
用于超灵敏即时检验的生物传感器需要干燥的试剂，这些试剂必须具有长期的稳定性和很高的信噪比。尽管邻位取代的二芳族二羟基和氨基羟基化合物会经历快速的氧化还原反应，但由于它们易于被溶解的氧氧化和聚合，因此不能用作电化学信号物质。在此报告中，稳定的固态1-氨基-2-萘基磷酸（1A2N-P）和氨硼烷（H 3 N-BH 3）分别用作碱性磷酸酶（ALP）的底物和电化学还原的还原剂。化学（EC）氧化还原循环。ALP将1A2N-P转化为1-氨基-2-萘酚（1A2N），然后使用H 3 N-BH 3将其用于EC氧化还原循环中。在H 3 N-BH 3的存在下，可明显防止1A2N被溶解的氧氧化和聚合。进行电化学测量时，无需用电催化材料修饰氧化铟锡（ITO）电极。为了进行比较，评估了包括1A2N在内的9种芳香族二羟基和氨基羟基化合物以实现快速的EC氧化还原循环，并且评估了包括H 3 N-BH 3在内的4种强还原剂以实现较低的背景水平。1A2N和H 3 N-BH 3的组合可以实现很高的信噪比。当新开发的组合用于肌酸激酶-MB（CK-MB）的检测时，CK-MB的检出限为〜80 fg / mL，表明该组合允许超灵敏的检测。使用开发的系统测定的临床血清样品中的CK-MB浓度与使用商业仪器获得的浓度高度吻合。因此，将稳定，固态的1A2N-P和H 3 N-BH 3与裸露的ITO电极一起使用对于超灵敏和简单的即时检测非常有希望。
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