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In vitro and in vivo analysis of visible light crosslinkable gelatin methacryloyl (GelMA) hydrogels
Biomaterials Science ( IF 5.8 ) Pub Date : 2017-07-04 00:00:00 , DOI: 10.1039/c7bm00110j Iman Noshadi 1 , Seonki Hong , Kelly E Sullivan , Ehsan Shirzaei Sani , Roberto Portillo-Lara , Ali Tamayol , Su Ryon Shin , Albert E Gao , Whitney L Stoppel , Lauren D Black , Ali Khademhosseini , Nasim Annabi
Biomaterials Science ( IF 5.8 ) Pub Date : 2017-07-04 00:00:00 , DOI: 10.1039/c7bm00110j Iman Noshadi 1 , Seonki Hong , Kelly E Sullivan , Ehsan Shirzaei Sani , Roberto Portillo-Lara , Ali Tamayol , Su Ryon Shin , Albert E Gao , Whitney L Stoppel , Lauren D Black , Ali Khademhosseini , Nasim Annabi
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Photocrosslinkable materials have been frequently used for constructing soft and biomimetic hydrogels for tissue engineering. Although ultraviolet (UV) light is commonly used for photocrosslinking such materials, its use has been associated with several biosafety concerns such as DNA damage, accelerated aging of tissues, and cancer. Here we report an injectable visible light crosslinked gelatin-based hydrogel for myocardium regeneration. Mechanical characterization revealed that the compressive moduli of the engineered hydrogels could be tuned in the range of 5–56 kPa by changing the concentrations of the initiator, co-initiator and co-monomer in the precursor formulation. In addition, the average pore sizes (26–103 μm) and swelling ratios (7–13%) were also shown to be tunable by varying the hydrogel formulation. In vitro studies showed that visible light crosslinked GelMA hydrogels supported the growth and function of primary cardiomyocytes (CMs). In addition, the engineered materials were shown to be biocompatible in vivo, and could be successfully delivered to the heart after myocardial infarction in an animal model to promote tissue healing. The developed visible light crosslinked hydrogel could be used for the repair of various soft tissues such as the myocardium and for the treatment of cardiovascular diseases with enhanced therapeutic functionality.
中文翻译：
可见光交联明胶甲基丙烯酰 (GelMA) 水凝胶的体外和体内分析
可光交联材料经常用于构建用于组织工程的软仿生水凝胶。尽管紫外线 (UV) 光通常用于光交联此类材料，但其使用与多种生物安全问题相关，例如 DNA 损伤、组织加速老化和癌症。在这里，我们报告了一种用于心肌再生的可注射可见光交联明胶基水凝胶。机械表征表明，通过改变前体配方中引发剂、共引发剂和共聚单体的浓度，可以将工程水凝胶的压缩模量调整在 5-56 kPa 的范围内。此外，平均孔径（26-103 μm）和溶胀率（7-13%）也可以通过改变水凝胶配方来调节。体外研究表明，可见光交联的 GelMA 水凝胶支持原代心肌细胞 (CM) 的生长和功能。此外，该工程材料在体内被证明具有生物相容性，并且可以在动物模型心肌梗塞后成功递送至心脏，以促进组织愈合。所开发的可见光交联水凝胶可用于修复心肌等多种软组织以及治疗心血管疾病，并增强治疗功能。
更新日期：2017-08-14
中文翻译：
可见光交联明胶甲基丙烯酰 (GelMA) 水凝胶的体外和体内分析
可光交联材料经常用于构建用于组织工程的软仿生水凝胶。尽管紫外线 (UV) 光通常用于光交联此类材料，但其使用与多种生物安全问题相关，例如 DNA 损伤、组织加速老化和癌症。在这里，我们报告了一种用于心肌再生的可注射可见光交联明胶基水凝胶。机械表征表明，通过改变前体配方中引发剂、共引发剂和共聚单体的浓度，可以将工程水凝胶的压缩模量调整在 5-56 kPa 的范围内。此外，平均孔径（26-103 μm）和溶胀率（7-13%）也可以通过改变水凝胶配方来调节。体外研究表明，可见光交联的 GelMA 水凝胶支持原代心肌细胞 (CM) 的生长和功能。此外，该工程材料在体内被证明具有生物相容性，并且可以在动物模型心肌梗塞后成功递送至心脏，以促进组织愈合。所开发的可见光交联水凝胶可用于修复心肌等多种软组织以及治疗心血管疾病，并增强治疗功能。
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