Graphene Field‐Effect Transistors for the Sensitive and Selective Detection of Escherichia coli Using Pyrene‐Tagged DNA Aptamer,Advanced Healthcare Materials

当前位置： X-MOL 学术 › Adv. Healthcare Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Graphene Field‐Effect Transistors for the Sensitive and Selective Detection of Escherichia coli Using Pyrene‐Tagged DNA Aptamer
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-08-10 , DOI: 10.1002/adhm.201700736
Guangfu Wu ₁ , Ziwen Dai ₂ , Xin Tang ₁ , Zihong Lin ₁ , Pik Kwan Lo ₂ , M. Meyyappan ₃ , King Wai Chiu Lai ₁

Affiliation

This study reports biosensing using graphene field‐effect transistors with the aid of pyrene‐tagged DNA aptamers, which exhibit excellent selectivity, affinity, and stability for Escherichia coli (E. coli) detection. The aptamer is employed as the sensing probe due to its advantages such as high stability and high affinity toward small molecules and even whole cells. The change of the carrier density in the probe‐modified graphene due to the attachment of E. coli is discussed theoretically for the first time and also verified experimentally. The conformational change of the aptamer due to the binding of E. coli brings the negatively charged E. coli close to the graphene surface, increasing the hole carrier density efficiently in graphene and achieving electrical detection. The binding of negatively charged E. coli induces holes in graphene, which are pumped into the graphene channel from the contact electrodes. The carrier mobility, which correlates the gate voltage to the electrical signal of the APG‐FETs, is analyzed and optimized here. The excellent sensing performance such as low detection limit, high sensitivity, outstanding selectivity and stability of the graphene biosensor for E. coli detection paves the way to develop graphene biosensors for bacterial detection.

中文翻译：

石墨烯场效应晶体管，用于使用P标记的DNA适体敏感和选择性地检测大肠杆菌

这项研究报告了在graph标记的DNA适体的帮助下，使用石墨烯场效应晶体管进行生物传感，该适体对大肠杆菌（E. coli）的检测具有出色的选择性，亲和性和稳定性。适体由于具有诸如对小分子甚至整个细胞的高稳定性和高亲和力的优点而被用作感测探针。由于大肠杆菌的附着，探针修饰的石墨烯中载流子密度的变化在理论上是首次讨论，并且也通过实验进行了验证。由于大肠杆菌的结合，适体的构象变化使带负电的大肠杆菌靠近石墨烯表面，有效地提高了石墨烯中的空穴载流子密度并实现了电检测。带负电的大肠杆菌的结合在石墨烯中诱导出空穴，该空穴从接触电极泵入石墨烯通道。在此分析和优化了将栅极电压与APG-FET的电信号相关的载流子迁移率。石墨烯生物传感器在大肠杆菌检测中的出色检测性能，如低检测限，高灵敏度，出色的选择性和稳定性，为开发用于细菌检测的石墨烯生物传感器铺平了道路。

更新日期：2017-08-10

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11