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Nanoneedle-Based Sensing in Biological Systems
ACS Sensors ( IF 8.2 ) Pub Date : 2017-08-08 00:00:00 , DOI: 10.1021/acssensors.7b00350
Ciro Chiappini 1
Affiliation  

Nanoneedles are high aspect ratio nanostructures with a unique biointerface. Thanks to their peculiar yet poorly understood interaction with cells, they very effectively sense intracellular conditions, typically with lower toxicity and perturbation than traditionally available probes. Through long-term, reversible interfacing with cells, nanoneedles can monitor biological functions over the course of several days. Their nanoscale dimension and the assembly into large-scale, ordered, dense arrays enable monitoring the functions of large cell populations, to provide functional maps with submicron spatial resolution. Intracellularly, they sense electrical activity of complex excitable networks, as well as concentration, function, and interaction of biomolecules in situ, while extracellularly they can measure the forces exerted by cells with piconewton detection limits, or efficiently sort rare cells based on their membrane receptors. Nanoneedles can investigate the function of many biological systems, ranging from cells, to biological fluids, to tissues and living organisms. This review examines the devices, strategies, and workflows developed to use nanoneedles for sensing in biological systems.

中文翻译:

生物系统中基于纳米针的传感

Nanoneedles是具有独特生物界面的高长径比纳米结构。由于它们与细胞的独特但尚未充分了解的相互作用,它们非常有效地感知了细胞内条件,与传统的探针相比,它们通常具有较低的毒性和干扰性。通过与细胞的长期可逆接口,纳米针可以在几天的过程中监控其生物学功能。它们的纳米级尺寸以及组装成大规模,有序,致密的阵列,使得能够监控大型细胞群的功能,从而提供具有亚微米空间分辨率的功能图。在细胞内,他们感知复杂的可兴奋网络的电活动,以及原位生物分子的浓度,功能和相互作用,而在细胞外,它们可以测量具有微微网检测极限的细胞所施加的力,或根据其膜受体有效地对稀有细胞进行分类。Nanoneedles可以研究许多生物系统的功能,包括细胞,生物流体,组织和活生物体。这篇综述检查了为使用纳米针在生物系统中进行传感而开发的设备,策略和工作流程。
更新日期:2017-08-08
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