Murine cutaneous leishmaniasis investigated by MALDI mass spectrometry imaging,Molecular BioSystems

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Murine cutaneous leishmaniasis investigated by MALDI mass spectrometry imaging
Molecular BioSystems Pub Date : 2017-07-27 00:00:00 , DOI: 10.1039/c7mb00411g
Fernanda Negrão _{1,

2,

3,

4,

5} , Daniele F. de O. Rocha _{1,

2,

3,

4} , Caroline F. Jaeeger _{1,

2,

3,

4} , Francisca J. S. Rocha _{4,

6,

7,

8} , Marcos N. Eberlin _{1,

2,

3,

4} , Selma Giorgio _{3,

4,

5,

9,

10}

Affiliation

Imaging mass spectrometry (IMS) is recognized as a powerful tool to investigate the spatial distribution of untargeted or targeted molecules of a wide variety of samples including tissue sections. Leishmania is a protozoan parasite that causes different clinical manifestations in mammalian hosts. Leishmaniasis is a major public health risk in different continents and represents one of the most important neglected diseases. Cutaneous lesions from mice experimentally infected with Leishmania spp. were investigated by matrix-assisted laser desorption ionization MS using the SCiLS Lab software for statistical analysis. Being applied to cutaneous leishmaniasis (CL) for the first time, MALDI-IMS was used to search for peptides and low molecular weight proteins (2–10 kDa) as candidates for potential biomarkers. Footpad sections of Balb/c mice infected with (i) Leishmania amazonensis or (ii) Leishmania major were imaged. The comparison between healthy and infected skin highlighted a set of twelve possible biomarker proteins for L. amazonenis and four proteins for L. major. Further characterization of these proteins could reveal how these proteins act in pathology progression and confirm their values as biomarkers.

中文翻译：

MALDI质谱成像研究小鼠皮肤利什曼病

成像质谱（IMS）被认为是研究包括组织切片在内的各种样本的未靶向或靶向分子的空间分布的有力工具。利什曼原虫是一种原生动物寄生虫，在哺乳动物宿主中引起不同的临床表现。利什曼病是不同大陆的主要公共卫生风险，是最重要的被忽视疾病之一。实验性感染利什曼原虫的小鼠的皮肤病变spp。通过使用SCiLS Lab软件进行基质分析的基质辅助激光解吸电离质谱仪进行了统计分析。MALDI-IMS首次被应用于皮肤利什曼病（CL），被用于寻找肽和低分子量蛋白质（2-10 kDa）作为潜在生物标志物的候选物。对感染（i）亚马逊利什曼原虫或（ii）大利什曼原虫的Balb / c小鼠的足垫切片成像。健康皮肤和感染皮肤之间的比较突出了一组十二脉线虫的生物标志物蛋白和大型乳酸菌的四种蛋白。这些蛋白质的进一步表征可以揭示这些蛋白质如何在病理学进程中起作用，并确认其作为生物标志物的价值。

更新日期：2017-08-07

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