Liposome‐Indocyanine Green Nanoprobes for Optical Labeling and Tracking of Human Mesenchymal Stem Cells Post‐Transplantation In Vivo,Advanced Healthcare Materials

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Liposome‐Indocyanine Green Nanoprobes for Optical Labeling and Tracking of Human Mesenchymal Stem Cells Post‐Transplantation In Vivo
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-08-04 , DOI: 10.1002/adhm.201700374
Mariarosa Mazza ₁ , Neus Lozano ₁ , Debora Braga Vieira ₁ , Maurizio Buggio ₁ , Cay Kielty ₂ , Kostas Kostarelos ₁

Affiliation

Direct labeling of human mesenchymal stem cells (hMSC) prior to transplantation provides a means to track cells after administration and it is a powerful tool for the assessment of new cell‐based therapies. Biocompatible nanoprobes consisting of liposome‐indocyanine green hybrid vesicles (liposome‐ICG) are used to safely label hMSC. Labeled hMSC recapitulating a 3D cellular environment is transplanted as spheroids subcutaneously and intracranially in athymic nude mice. Cells emit a strong NIR signal used for tracking post‐transplantation with the IVIS imaging system up to 2 weeks (subcutaneous) and 1 week (intracranial). The transplanted stem cells are imaged in situ after engraftment deep in the brain up to 1 week in living animals using optical imaging techniques and without the need to genetically modify the cells. This method is proposed for efficient, nontoxic direct cell labeling for the preclinical assessment of cell‐based therapies and the design of clinical trials, and potentially for localization of the cell engraftment after transplantation into patients.

中文翻译：

用于体内移植后人间充质干细胞光学标记和跟踪的脂质体-吲哚菁绿纳米探针

移植前直接标记人间充质干细胞 (hMSC) 提供了一种在给药后跟踪细胞的方法，它是评估基于细胞的新疗法的有力工具。由脂质体-吲哚菁绿杂化囊泡（脂质体-ICG）组成的生物相容性纳米探针用于安全标记hMSC。将重现 3D 细胞环境的标记 hMSC 作为球体移植到无胸腺裸鼠的皮下和颅内。细胞发出强烈的 NIR 信号，用于使用 IVIS 成像系统跟踪移植后长达 2 周（皮下）和 1 周（颅内）。移植的干细胞在植入活体动物大脑深处长达 1 周后使用光学成像技术进行原位成像，无需对细胞进行基因改造。

更新日期：2017-08-04

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