As an outstanding candidate of molecular logic computing, DNA logic computing has gained extensive advancements across diverse research areas. Nevertheless, current DNA logic gates with various functions, are fragmentary nominated and always separately constructed in the light of their different operating-principles. The tedious/obligatory gates’ redesign/reoperation brought longer time, higher costs and lower computing efficiency. Herein, we for the first time proposed the concept “Contrary Logic Pairs” to systematically classify DNA gates with opposite functions into “positiveΛnegative” gates (CLP=PosΛNeg). By utilizing two fluorescent substrates (Amplex Red, Scopoletin) of G-quadruplex DNAzyme as label-free signal-reporters and based on solely DNA hybridization, we fabricated the first intelligent universal system that “yield double results with half the effort” for engineering a DNA CLPs library and various DNA combinatorial logic circuits. Different from previous DNA logic systems, as for the non-interference between two substrates, “PosΛNeg” gates of each DNA CLP in this system were operated via the same DNA reaction at one time, without gates’ redesign/reoperation. With the modulation of DNA reactions, a DNA CLPs library was fabricated. More amazingly, through switching the selective/parallel operating-mode, “PosΛNeg” gates of each CLP could not only be alternatively constructed, but also be integrated into DNA combinatorial logic circuits (Pos/p/Neg). All the strategies largely simplified the operation, reduced the time/costs of current DNA gates’ construction by at least 1/2, accompanied with obviously improved computing efficiency. Furthermore, a DNA voter with “One-Vote Deny” function that executed by multiple equal deniers was realized.

中文翻译：

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一种智能通用系统，“用一半的力气就能获得双倍的回报”，用于设计DNA“相反逻辑对”库和各种DNA组合逻辑电路

作为分子逻辑计算的杰出候选人，DNA逻辑计算在各个研究领域都取得了长足的进步。然而，目前具有各种功能的DNA逻辑门是碎片提名的，并且总是根据它们的不同操作原理分别构建。繁琐/强制性的闸门的重新设计/重新操作带来了更长的时间，更高的成本和更低的计算效率。在此，我们首次提出了“相反逻辑对”的概念，将具有相反功能的DNA门系统地分类为“正”-“负”门（CLP = Pos ^ Neg）。通过将G-四链体DNAzyme的两种荧光底物（Amplex Red，Scopoletin）用作无标记信号报告物，并且仅基于DNA杂交，我们制造了第一个智能通用系统，“用一半的努力就能获得双倍的结果”，用于设计DNA CLP库和各种DNA组合逻辑电路。与以前的DNA逻辑系统不同，关于两个基板之间的互不干扰，该系统中每个DNA CLP的“PosΛNeg”门都是通过相同的DNA反应同时操作的，而无需重新设计/重新操作门。通过调节DNA反应，制备了DNA CLPs文库。更令人惊讶的是，通过切换选择性/并行操作模式，每个CLP的“PosΛNeg”门不仅可以交替构建，而且可以集成到DNA组合逻辑电路（Pos / p / Neg）中。所有策略在很大程度上简化了操作，将当前DNA Gate的构建时间/成本降低了至少1/2，伴随着明显提高的计算效率。此外，实现了具有“一票否决”功能的DNA投票器，该功能由多个相等的旦尼尔执行。