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The combined effects of silicon dioxide nanoparticles and cold air exposure on the metabolism and inflammatory responses in white adipocytes
Toxicology Research ( IF 2.2 ) Pub Date : 2017-07-06 00:00:00 , DOI: 10.1039/c7tx00145b Yongqiang Zhang 1, 2, 3 , Xi Li 1, 2, 3 , Yangsheng Lin 1, 2, 3 , Li Zhang 1, 2, 3 , Zhan Guo 1, 2, 3 , Dandan Zhao 1, 2, 3 , Danfeng Yang 1, 2, 3
Toxicology Research ( IF 2.2 ) Pub Date : 2017-07-06 00:00:00 , DOI: 10.1039/c7tx00145b Yongqiang Zhang 1, 2, 3 , Xi Li 1, 2, 3 , Yangsheng Lin 1, 2, 3 , Li Zhang 1, 2, 3 , Zhan Guo 1, 2, 3 , Dandan Zhao 1, 2, 3 , Danfeng Yang 1, 2, 3
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The potential hazard of nanoparticles (NPs) from air pollution has attracted widespread attention. Though the toxicology of NPs has been intensively studied, few works have been reported on the combined effect of silicon dioxide (SiO2) NPs and cold air exposure at the cellular level. Herein, we evaluated the combined effect of SiO2 NPs and cold exposure on metabolism and the inflammatory responses in white adipocytes by qRT-PCR in vitro. After SiO2 NP or cold exposure, there were significant changes in the expressions of adipogenic genes and proinflammatory cytokine genes in white adipocytes. The mRNA levels of IL-6, IL-8, TNF-α and IL-1β were upregulated by SiO2 NP or cold exposure, and more so in the combined group. The expressions of the proinflammatory cytokine genes IL-6, IL-8, TNF-α and IL-1β increased highly significantly (P < 0.01) in the SiO2 NP alone group and the combined group, compared with the control. The expressions of the cold group tended to be upregulated significantly compared with the control in IL-6 (P < 0.01) and IL-8 (P < 0.05). The results demonstrated that there was antagonistic effect between SiO2 NPs and cold air on the plasticity and metabolism in white adipocytes, where the main effect of cold air on the plasticity and metabolism was significant (P < 0.05). However, there was a synergistic effect between SiO2 NPs and cold air on the toxic effects in white adipocytes, in which the main effect of SiO2 NPs on the toxic effects was significant (P < 0.05). In conclusion, SiO2 NPs combined with cold exposure induced a stronger inflammatory response and influenced the plasticity and metabolism in white adipocytes, accompanied by more serious health hazards.
中文翻译：
二氧化硅纳米颗粒和冷空气暴露对白色脂肪细胞代谢和炎症反应的综合影响
空气污染引起的纳米颗粒（NPs）的潜在危害已引起广泛关注。尽管已经对NPs的毒理学进行了深入研究，但是关于二氧化硅（SiO 2）NPs和在细胞水平上暴露于冷空气的综合作用的报道很少。在本文中，我们评估的SiO的组合效果2的NP和代谢冷曝光和在白色脂肪细胞的炎症反应通过qRT-PCR在体外。SiO 2 NP或冷暴露后，白色脂肪细胞中成脂基因和促炎细胞因子基因的表达发生了显着变化。SiO 2上调IL-6，IL-8，TNF-α和IL-1β的mRNA水平。NP或冷暴露，在合并组中更是如此。与对照组相比，单独的SiO 2 NP组和联合组中促炎细胞因子基因IL-6，IL-8，TNF-α和IL-1β的表达显着增加（P <0.01）。IL-6（P <0.01）和IL-8（P <0.05）与对照组相比，寒冷组的表达有明显上调的趋势。结果表明，SiO 2 NPs和冷空气对白色脂肪细胞的可塑性和代谢具有拮抗作用，其中冷空气对可塑性和代谢的主要影响是显着的（P <0.05）。但是，SiO之间有协同作用2 NPs和冷空气对白色脂肪细胞的毒性作用，其中SiO 2 NPs对毒性的主要作用是显着的（ P <0.05）。总之，SiO 2 NPs结合冷暴露可引起更强的炎症反应，并影响白色脂肪细胞的可塑性和新陈代谢，同时对健康的危害更为严重。
更新日期：2017-08-03
中文翻译：
二氧化硅纳米颗粒和冷空气暴露对白色脂肪细胞代谢和炎症反应的综合影响
空气污染引起的纳米颗粒（NPs）的潜在危害已引起广泛关注。尽管已经对NPs的毒理学进行了深入研究，但是关于二氧化硅（SiO 2）NPs和在细胞水平上暴露于冷空气的综合作用的报道很少。在本文中，我们评估的SiO的组合效果2的NP和代谢冷曝光和在白色脂肪细胞的炎症反应通过qRT-PCR在体外。SiO 2 NP或冷暴露后，白色脂肪细胞中成脂基因和促炎细胞因子基因的表达发生了显着变化。SiO 2上调IL-6，IL-8，TNF-α和IL-1β的mRNA水平。NP或冷暴露，在合并组中更是如此。与对照组相比，单独的SiO 2 NP组和联合组中促炎细胞因子基因IL-6，IL-8，TNF-α和IL-1β的表达显着增加（P <0.01）。IL-6（P <0.01）和IL-8（P <0.05）与对照组相比，寒冷组的表达有明显上调的趋势。结果表明，SiO 2 NPs和冷空气对白色脂肪细胞的可塑性和代谢具有拮抗作用，其中冷空气对可塑性和代谢的主要影响是显着的（P <0.05）。但是，SiO之间有协同作用2 NPs和冷空气对白色脂肪细胞的毒性作用，其中SiO 2 NPs对毒性的主要作用是显着的（ P <0.05）。总之，SiO 2 NPs结合冷暴露可引起更强的炎症反应，并影响白色脂肪细胞的可塑性和新陈代谢，同时对健康的危害更为严重。
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