当前位置: X-MOL 学术Metallomics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
BRUTUS and its paralogs, BTS LIKE1 and BTS LIKE2, encode important negative regulators of the iron deficiency response in Arabidopsis thaliana†
Metallomics ( IF 2.9 ) Pub Date : 2017-06-16 00:00:00 , DOI: 10.1039/c7mt00152e
Maria N Hindt 1 , Garo Z Akmakjian , Kara L Pivarski , Tracy Punshon , Ivan Baxter , David E Salt , Mary Lou Guerinot
Affiliation  

Iron (Fe) is required for plant health, but it can also be toxic when present in excess. Therefore, Fe levels must be tightly controlled. The Arabidopsis thaliana E3 ligase BRUTUS (BTS) is involved in the negative regulation of the Fe deficiency response and we show here that the two A. thaliana BTS paralogs, BTS LIKE1 (BTSL1) and BTS LIKE2 (BTSL2) encode proteins that act redundantly as negative regulators of the Fe deficiency response. Loss of both of these E3 ligases enhances tolerance to Fe deficiency. We further generated a triple mutant with loss of both BTS paralogs and a partial loss of BTS expression that exhibits even greater tolerance to Fe-deficient conditions and increased Fe accumulation without any resulting Fe toxicity effects. Finally, we identified a mutant carrying a novel missense mutation of BTS that exhibits an Fe deficiency response in the root when grown under both Fe-deficient and Fe-sufficient conditions, leading to Fe toxicity when plants are grown under Fe-sufficient conditions.

中文翻译:


BRUTUS 及其旁系同源物 BTS LIKE1 和 BTS LIKE2 编码拟南芥缺铁反应的重要负调节因子†



铁 (Fe) 是植物健康所必需的,但过量时也可能有毒。因此,必须严格控制铁含量。拟南芥E3 连接酶 BRUTUS (BTS) 参与缺铁反应的负调节,我们在此表明​​两个拟南芥 BTS旁系同源物BTS LIKE1 ( BTSL1 ) 和BTS LIKE 2 ( BTSL2 ) 编码冗余作用的蛋白质作为铁缺乏反应的负调节剂。失去这两种 E3 连接酶会增强对缺铁的耐受性。我们进一步产生了一种三重突变体,其同时丧失了BTS旁系同源物和部分丧失了BTS表达,该突变体对缺铁条件表现出更大的耐受性并增加了铁积累,而没有任何由此产生的铁毒性作用。最后,我们鉴定了一种携带新的BTS错义突变的突变体,当在缺铁和铁充足条件下生长时,该突变体在根中表现出缺铁反应,导致植物在铁充足条件下生长时产生铁毒性。
更新日期:2017-06-16
down
wechat
bug