当前位置：
X-MOL 学术
›
ACS Macro Lett.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Sustained Release of Recombinant Human Growth Hormone from Bioresorbable Poly(ester urea) Nanofibers
ACS Macro Letters ( IF 5.1 ) Pub Date : 2017-08-02 00:00:00 , DOI: 10.1021/acsmacrolett.7b00334 Yaohua Gao 1 , Yanyi Xu 1 , Adam Land 2 , Justin Harris 1 , Gina M. Policastro 1 , Erin P. Childers 1 , Todd Ritzman 3 , Joshua Bundy 1 , Matthew L. Becker 1
ACS Macro Letters ( IF 5.1 ) Pub Date : 2017-08-02 00:00:00 , DOI: 10.1021/acsmacrolett.7b00334 Yaohua Gao 1 , Yanyi Xu 1 , Adam Land 2 , Justin Harris 1 , Gina M. Policastro 1 , Erin P. Childers 1 , Todd Ritzman 3 , Joshua Bundy 1 , Matthew L. Becker 1
Affiliation
|
Recombinant human growth hormone (rhGH) has been used clinically for several decades. However, rhGH has a short half-life in vivo and therefore for efficacy requires frequent subcutaneous injection, leading to pain and poor patient compliance. An effective rhGH delivery system able to continuously release rhGH over a comparatively long period (generally over a month) would be a significant clinical advance. In this study, a novel long-duration release strategy of rhGH was developed by encapsulating sugar glass-stabilized rhGH in electrospun, bioresorbable poly(ester urea) (PEU) nanofibers. The rhGH was found to be randomly dispersed among the fibers, and sustained rhGH release with only a modest burst was observed for at least 6 weeks as studied by a bicinchoninic acid (BCA) protein assay. Significantly, the released rhGH remained bioavailable and bioactive as determined by an Nb2 cell bioassay specific for rhGH. Our results suggest the feasibility of this system as an effective long-term sustained release strategy for rhGH and potentially other therapeutic proteins.
中文翻译：
从生物可吸收聚酯（酯尿素）纳米纤维中持续释放重组人生长激素。
重组人生长激素（rhGH）在临床上已经使用了几十年。但是，rhGH在体内的半衰期很短因此，为了获得疗效，需要频繁地皮下注射，从而导致疼痛和患者依从性差。能够在相对较长的时间内（通常一个月以上）连续释放rhGH的有效rhGH递送系统将是一项重大的临床进展。在这项研究中，通过将糖玻璃稳定的rhGH封装在静电纺丝，可生物吸收的聚（酯脲）（PEU）纳米纤维中，开发了一种新型的rhGH持久释放策略。发现rhGH随机分散在纤维中，并且通过二辛可宁酸（BCA）蛋白测定法研究，在至少6周内观察到持续的rhGH释放，只有适度的爆发。值得注意的是，释放的rhGH仍具有生物利用度和生物活性，这是通过对rhGH特异的Nb2细胞生物测定法确定的。
更新日期：2017-08-02
中文翻译：
从生物可吸收聚酯（酯尿素）纳米纤维中持续释放重组人生长激素。
重组人生长激素（rhGH）在临床上已经使用了几十年。但是，rhGH在体内的半衰期很短因此，为了获得疗效，需要频繁地皮下注射，从而导致疼痛和患者依从性差。能够在相对较长的时间内（通常一个月以上）连续释放rhGH的有效rhGH递送系统将是一项重大的临床进展。在这项研究中，通过将糖玻璃稳定的rhGH封装在静电纺丝，可生物吸收的聚（酯脲）（PEU）纳米纤维中，开发了一种新型的rhGH持久释放策略。发现rhGH随机分散在纤维中，并且通过二辛可宁酸（BCA）蛋白测定法研究，在至少6周内观察到持续的rhGH释放，只有适度的爆发。值得注意的是，释放的rhGH仍具有生物利用度和生物活性，这是通过对rhGH特异的Nb2细胞生物测定法确定的。
京公网安备 11010802027423号