Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code,ChemBioChem

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Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code
ChemBioChem ( IF 2.6 ) Pub Date : 2017-08-01 09:15:51 , DOI: 10.1002/cbic.201700327
Matthias Hauf ₁ , Florian Richter ₂ , Tobias Schneider ₁ , Thomas Faidt ₃ , Berta M. Martins ₄ , Tobias Baumann ₁ , Patrick Durkin ₁ , Holger Dobbek ₄ , Karin Jacobs ₃ , Andreas Möglich _{2,

5} , Nediljko Budisa ₁

Affiliation

Marine mussels exhibit potent underwater adhesion abilities under hostile conditions by employing 3,4-dihydroxyphenylalanine (DOPA)-rich mussel adhesive proteins (MAPs). However, their recombinant production is a major biotechnological challenge. Herein, a novel strategy based on genetic code expansion has been developed by engineering efficient aminoacyl-transfer RNA synthetases (aaRSs) for the photocaged noncanonical amino acid ortho-nitrobenzyl DOPA (ONB-DOPA). The engineered ONB-DOPARS enables in vivo production of MAP type 5 site-specifically equipped with multiple instances of ONB-DOPA to yield photocaged, spatiotemporally controlled underwater adhesives. Upon exposure to UV light, these proteins feature elevated wet adhesion properties. This concept offers new perspectives for the production of recombinant bioadhesives.

中文翻译：

通过扩展的遗传密码可光活化基于贻贝的水下粘附蛋白

通过使用富含3,4-二羟基苯丙氨酸（DOPA）的贻贝粘附蛋白（MAPs），海洋贻贝在敌对条件下表现出强大的水下粘附能力。然而，它们的重组生产是主要的生物技术挑战。在本文中，已经通过工程化用于光笼型非规范氨基酸邻位的有效的氨酰基转移RNA合成酶（aaRSs），开发了一种基于遗传密码扩展的新策略。-硝基苄基DOPA（ONB-DOPA）。经过工程设计的ONB-DOPARS可实现体内特定位置配备多种ONB-DOPA实例的MAP类型5的体内生产，从而产生光笼封，时空控制的水下胶粘剂。暴露于紫外线后，这些蛋白质的湿粘附特性提高。这一概念为重组生物粘合剂的生产提供了新的视角。

更新日期：2017-08-02

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