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A Boronic Acid Assay for the Detection of Mucin-1 Glycoprotein from Cancer Cells
ChemBioChem ( IF 2.6 ) Pub Date : 2017-08-01 06:11:09 , DOI: 10.1002/cbic.201700396
Xiaoyu Zhang 1 , Shiqiang Zhang 2 , Seung Joon Baek 3 , Michael D. Best 1
Affiliation  

The back cover picture shows a sensitive, high-throughput platform for the detection of glycoproteins that are aberrantly expressed in disease, such as cancer-associated mucin-1. This assay, which enables the detection of mucin-1 directly from cancer cell lysates, entails the immobilization of carbohydrate-binding boronic acid moieties onto microplates coupled with chemiluminescence-based antibody analysis of surface-bound glycoproteins. More information can be found in the communication by M. D. Best et al. (DOI: 10.1002/cbic.201700288).

中文翻译:

硼酸从癌细胞中检测粘蛋白1糖蛋白的含量

封底图片显示了一个灵敏的高通量平台,用于检测在疾病(例如与癌症相关的粘蛋白1)中异常表达的糖蛋白。这种测定法能够直接从癌细胞裂解物中检测粘蛋白1,它需要将结合碳水化合物的硼酸部分固定在微板上,并结合基于化学发光的表面结合糖蛋白抗体分析。可以在M. D. Best等人的来文中找到更多信息。(DOI:10.1002 / cbic.201700288)。
更新日期:2017-08-02
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