Cyanine (Cy) dyes show a general propensity to localize in polarized mitochondria. This mitochondriotropism was used to perform a copper-free click reaction in the mitochondria of living cells. The in organello reaction of dyes Cy3 and Cy5 led to a product that was easily traceable by Förster resonance energy transfer (FRET). As determined by confocal laser scanning microscopy, the Cy3–Cy5 conjugate showed enhanced retention in mitochondria, relative to that of the starting compounds. This enhancement of a favorable property can be achieved by synthesis in organello, but not outside mitochondria.

中文翻译：

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Organello无铜点击反应对花菁染料线粒体性质的调控

花青（Cy）染料显示出普遍存在于极化线粒体中的倾向。该线粒体向性用于在活细胞的线粒体中进行无铜点击反应。染料Cy3和Cy5的器官内反应产生了易于通过Förster共振能量转移（FRET）追溯的产物。通过共聚焦激光扫描显微镜确定，相对于起始化合物，Cy3–Cy5共轭物在线粒体中的保留增强。可以通过在器皿中合成，但不在线粒体中合成来实现有利特性的增强。