MALDI MS imaging investigation of the host response to visceral leishmaniasis,Molecular BioSystems

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MALDI MS imaging investigation of the host response to visceral leishmaniasis
Molecular BioSystems Pub Date : 2017-07-20 00:00:00 , DOI: 10.1039/c7mb00306d
C. F. Jaegger _{1,

2,

3,

4} , F. Negrão _{1,

2,

3,

4} , D. M. Assis _{4,

5,

6} , K. R. A. Belaz _{1,

2,

3,

4} , C. F. F. Angolini _{1,

2,

3,

4} , A. M. A. P. Fernandes _{1,

2,

3,

4} , V. G. Santos _{1,

2,

3,

4} , A. Pimentel _{2,

3,

4,

7,

8} , D. R. Abánades _{2,

3,

4,

7,

8} , S. Giorgio _{2,

3,

4,

7,

8} , M. N. Eberlin _{1,

2,

3,

4} , D. F. O. Rocha _{1,

2,

3,

4}

Affiliation

Mass spectrometry imaging (MSI) of animal tissues has become an important tool for in situ molecular analyses and biomarker studies in several clinical areas, but there are few applications in parasitological studies. Leishmaniasis is a neglected tropical disease, and experimental mouse models have been essential to evaluate pathological and immunological processes and to develop diagnostic methods. Herein we have employed MALDI MSI to examine peptides and low molecular weight proteins (2 to 20 kDa) differentially expressed in the liver during visceral leishmaniasis in mice models. We analyzed liver sections of Balb/c mice infected with Leishmania infantum using the SCiLS Lab software for statistical analysis, which facilitated data interpretation and thus highlighted several key proteins and/or peptides. We proposed a decision tree classification for visceral leishmaniasis with distinct phases of the disease, which are named here as healthy, acute infection and chronic infection. Among others, the ion of m/z 4963 was the most important to identify acute infection and was tentatively identified as Thymosin β4. This peptide was previously established as a recovery factor in the human liver and might participate in the response of mice to Leishmania infection. This preliminary investigation shows the potential of MALDI MSI to complement classical compound selective imaging techniques and to explore new features not yet recognized by these approaches.

中文翻译：

MALDI MS成像研究宿主对内脏利什曼病的反应

动物组织的质谱成像（MSI）已成为一些临床领域中进行原位分子分析和生物标志物研究的重要工具，但在寄生虫学研究中却很少有应用。利什曼病是一种被忽视的热带病，实验性小鼠模型对于评估病理和免疫过程以及开发诊断方法至关重要。本文中，我们使用MALDI MSI来检查小鼠内脏利什曼病期间在肝脏中差异表达的肽和低分子量蛋白质（2至20 kDa）。我们分析了感染婴儿利什曼原虫的Balb / c小鼠的肝脏切片使用SCiLS Lab软件进行统计分析，这有助于数据解释，从而突出显示了几种关键的蛋白质和/或肽。我们提出了针对内脏利什曼病具有不同疾病阶段的决策树分类，在此将其命名为健康，急性感染和慢性感染。其中，m / z 4963的离子是识别急性感染最重要的离子，初步确定为胸腺素β4。该肽先前已被确定为人类肝脏的恢复因子，可能参与了小鼠对利什曼原虫的反应感染。这项初步研究表明，MALDI MSI可以补充经典的化合物选择性成像技术，并探索这些方法尚未认识到的新功能。

更新日期：2017-07-31

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