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Making the Message Clear: Concepts for mRNA Imaging
ACS Central Science ( IF 12.7 ) Pub Date : 2017-07-06 00:00:00 , DOI: 10.1021/acscentsci.7b00251
Kristina Rau 1 , Andrea Rentmeister 1
Affiliation  

The transcriptome of each individual cell contains numerous RNA species, each of which can be controlled by multiple mechanisms during their lifetime. The standard transcriptome analysis focuses on the expression levels of the genes of interest. To gain additional insights into spatiotemporal RNA distribution and the underlying trafficking processes, RNA labeling and imaging are necessary—ideally in living cells. This perspective will summarize state-of-the-art RNA imaging methods including their strengths and weaknesses.

中文翻译:

明确表达信息:mRNA成像的概念

每个单个细胞的转录组都包含许多RNA种类,在它们的生命周期中,每种都可以通过多种机制进行控制。标准的转录组分析关注目标基因的表达水平。为了对时空RNA分布和潜在的运输过程有更多的了解,RNA标记和成像是必要的-理想的是在活细胞中。该观点将总结最新的RNA成像方法,包括其优缺点。
更新日期:2017-07-28
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