Identification of Antiangiogenic Potential and Cellular Mechanisms of Napyradiomycin A1 Isolated from the Marine-Derived Streptomyces sp. YP127,Journal of Natural Products

当前位置： X-MOL 学术 › J. Nat. Prod. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Identification of Antiangiogenic Potential and Cellular Mechanisms of Napyradiomycin A1 Isolated from the Marine-Derived Streptomyces sp. YP127
Journal of Natural Products ( IF 3.3 ) Pub Date : 2017-07-27 00:00:00 , DOI: 10.1021/acs.jnatprod.7b00211
Ji Sun Hwang ₁ , Geum Jin Kim ₂ , Hyun Gyu Choi ₂ , Min Cheol Kim ₃ , Dongyup Hahn , Joo-Won Nam ₂ , Sang-Jip Nam ₄ , Hak Choel Kwon ₃ , Jungwook Chin ₁ , Sung Jin Cho _{1,

5} , Hayoung Hwang ₁ , Hyukjae Choi ₂

Affiliation

Angiogenesis is the process of new blood vessel formation. Excessive angiogenesis is a critical factor in the progression of cancer, macular degeneration, and other chronic inflammatory diseases. When investigating the effects of crude extracts of cultured marine microorganisms, an extract of the cultured Streptomyces sp. YP127 strain was found to inhibit human umbilical vein endothelial cell (HUVEC) tube formation. Bioassay-guided fractionation and spectroscopic data analyses led to the identification of napyradiomycin A1 (1) as an antiangiogenic component of the extract. Compound 1 inhibited HUVEC tube formation in a concentration-dependent manner. It inhibited endothelial cell proliferation but did not affect human dermal fibroblast proliferation. Compound 1 also suppressed migration and invasion of vascular endothelial cells. In addition, compound 1 suppressed vascular endothelial cadherin expression and increased the permeability of the endothelial cell membrane. These results suggested that compound 1 modulates cell permeability and inhibits the angiogenesis of endothelial cells.

中文翻译：

从海洋衍生链霉菌中分离得到的那比霉素A1的抗血管生成潜力和细胞机制的鉴定。YP127

血管生成是新血管形成的过程。过度的血管生成是癌症，黄斑变性和其他慢性炎症性疾病进展的关键因素。在调查海洋微生物培养物粗提物的影响时，应使用链霉菌属菌种的提取物。发现YP127菌株可抑制人脐静脉内皮细胞（HUVEC）管的形成。生物测定指导的分级分离和光谱数据分析导致鉴定出那不勒斯霉素A1（1）作为提取物中的抗血管生成成分。化合物1浓度依赖性抑制HUVEC管的形成。它抑制内皮细胞增殖，但不影响人类皮肤成纤维细胞增殖。化合物1还抑制血管内皮细胞的迁移和侵袭。另外，化合物1抑制血管内皮钙粘蛋白的表达并增加内皮细胞膜的通透性。这些结果表明化合物1调节细胞渗透性并抑制内皮细胞的血管生成。

更新日期：2017-07-27

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11