Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST),Chemical Communications

当前位置： X-MOL 学术 › Chem. Commun. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST)
Chemical Communications ( IF 4.3 ) Pub Date : 2017-07-07 00:00:00 , DOI: 10.1039/c7cc05021f
Qinglin Wu _{1,

2,

3,

4} , Benjamin A. Fenton _{1,

2,

3,

4} , Jessica L. Wojtaszek _{1,

2,

3,

4} , Pei Zhou _{1,

2,

3,

4}

Affiliation

CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HN^dec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HN^dec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations.

中文翻译：

通过氮解耦酰胺质子化学交换饱和转移（HN ^dec -CEST）探测激发态化学位移和交换参数

CEST-NMR光谱是探测大分子构象动力学的有力工具。我们提出了一种HN ^dec -CEST实验，该实验可简化松弛矩阵，减少拟合参数并增强信号分辨率。重要的是，HN ^dec -CEST曲线的拟合使得能够可靠地提取汇率以及激发态化学位移和化学势。

更新日期：2017-07-28

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11