Deciphering the Nature of Enzymatic Modifications of Bacterial Cell Walls,ChemBioChem

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Deciphering the Nature of Enzymatic Modifications of Bacterial Cell Walls
ChemBioChem ( IF 2.6 ) Pub Date : 2017-07-25 04:06:20 , DOI: 10.1002/cbic.201700293
Mijoon Lee ₁ , Dusan Hesek ₁ , Elena Lastochkin ₁ , David A. Dik ₁ , Bill Boggess ₁ , Shahriar Mobashery ₁

Affiliation

The major constituent of bacterial cell walls is peptidoglycan, which, in its crosslinked form, is a polymer of considerable complexity that encases the entire bacterium. A functional cell wall is indispensable for survival of the organism. There are several dozen enzymes that assemble and disassemble the peptidoglycan dynamically within each bacterial generation. Understanding of the nature of these transformations is critical knowledge for these events. Octasaccharide peptidoglycans were prepared and studied with seven recombinant cell-wall-active enzymes (SltB1, MltB, RlpA, mutanolysin, AmpDh2, AmpDh3, and PBP5). With the use of highly sensitive mass spectrometry methods, we described the breadth of reactions that these enzymes catalyzed with peptidoglycan and shed light on the nature of the cell wall alteration performed by these enzymes. The enzymes exhibit broadly distinct preferences for their substrate peptidoglycans in the reactions that they catalyze.

中文翻译：

破译细菌细胞壁酶促修饰的性质

细菌细胞壁的主要成分是肽聚糖，它的交联形式是一种非常复杂的聚合物，可以包裹整个细菌。功能性细胞壁对于生物的生存是必不可少的。在每个细菌世代中，有数十种酶可以动态地组装和分解肽聚糖。了解这些转换的本质是这些事件的关键知识。制备八糖肽聚糖，并用七种重组细胞壁活性酶（SltB1，MltB，RlpA，变溶菌素，AmpDh2，AmpDh3和PBP5）进行研究。通过使用高度敏感的质谱方法，我们描述了这些酶与肽聚糖催化反应的广度，并阐明了这些酶进行的细胞壁改变的性质。

更新日期：2017-07-26

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