Efficient Delivery of Quantum Dots into the Cytosol of Cells Using Cell-Penetrating Poly(disulfide)s,Journal of the American Chemical Society

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Efficient Delivery of Quantum Dots into the Cytosol of Cells Using Cell-Penetrating Poly(disulfide)s
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-07-25 , DOI: 10.1021/jacs.7b02952
Emmanuel Derivery ₁ , Eline Bartolami ₂ , Stefan Matile ₂ , Marcos Gonzalez-Gaitan ₂

Affiliation

Quantum dots (QDs) are extremely bright, photostable, nanometer particles broadly used to investigate single molecule dynamics in vitro. However, the use of QDs in vivo to investigate single molecule dynamics is impaired by the absence of an efficient way to chemically deliver them into the cytosol of cells. Indeed, current methods (using cell-penetrating peptides for instance) provide very low yields: QDs stay at the plasma membrane or are trapped in endosomes. Here, we introduce a technology based on cell-penetrating poly(disulfide)s that solves this problem: we deliver about 70 QDs per cell, and 90% appear to freely diffuse in the cytosol. Furthermore, these QDs can be functionalized, carrying GFP or anti-GFP nanobodies for instance. Our technology thus paves the way toward single molecule imaging in cells and living animals, allowing to probe biophysical properties of the cytosol.

中文翻译：

使用细胞穿透性聚（二硫化物）将量子点有效传递到细胞的胞质溶胶中

量子点 (QD) 是极其明亮、光稳定的纳米粒子，广泛用于体外研究单分子动力学。然而，由于缺乏一种有效的方法将它们化学递送到细胞的胞质溶胶中，因此在体内使用量子点来研究单分子动力学受到了损害。事实上，目前的方法（例如使用细胞穿透肽）提供的产量非常低：量子点停留在质膜或被困在内体中。在这里，我们介绍了一种基于细胞穿透性聚（二硫化物）的技术来解决这个问题：我们每个细胞提供大约 70 个 QD，90% 似乎在细胞质中自由扩散。此外，这些量子点可以进行功能化，例如携带 GFP 或抗 GFP 纳米抗体。因此，我们的技术为细胞和活体动物的单分子成像铺平了道路，

更新日期：2017-07-25

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