5-Formylcytosine Yields DNA–Protein Cross-Links in Nucleosome Core Particles,Journal of the American Chemical Society

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5-Formylcytosine Yields DNA–Protein Cross-Links in Nucleosome Core Particles
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-07-25 , DOI: 10.1021/jacs.7b05495
Fengchao Li ₁ , Yingqian Zhang ₁ , Jing Bai ₁ , Marc M. Greenberg ₂ , Zhen Xi _{1,

3} , Chuanzheng Zhou _{1,

3}

Affiliation

In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.

中文翻译：

5-甲酰胞嘧啶在核小体核心颗粒中产生 DNA-蛋白质交联

在核小体核心颗粒 (NCP) 中原位生成 5-甲酰基胞嘧啶 (5fC) 表明 5fC 导致必需的 DNA-蛋白质交联 (DPC)。使用化学模型和突变组蛋白的机理研究表明，DPC 在 5fC 的甲酰功能和组蛋白上的伯胺之间可逆地形成。这些结果表明，染色质中 5fC 的 DPC 形成除了其在 DNA 去甲基化中的作用外。

更新日期：2017-07-25

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