A new peptide nucleic acid (PNA) with an installed peroxidase function has been developed. Fmoc solid phase peptide synthesis prepared a PNA hybrid (VQKCAQCHTVE-(C₂H₄O)₂CH₂-[PNA(T)]₆-G) that renders the microperoxidase backbone, followed by reconstitution with hemin. The resulting holocompound catalyzed the oxidation of 3,3′,5,5′-tetramthylbenzidine by H₂O₂ to 50% that of natural microperoxidase-11, whereas the apo-form and hemin gave no responses. The peroxidase domain was found to be active toward direct electrochemistry and the PNA hybrid served for gene sensor; in the presence of the target DNA (5′-CATGTATAAAAAA-3′), an electrode-attached DNA probe (5′-TsTsTsTsTsTCTCATACATG-3′) showed the ferric-to-ferrous quasi-reversible wave (−276 mV vs Ag/AgCl) through sandwich hybridization. Moreover, the hybridization product could accept H₂O₂ as an oxidant to enhance the reduction current, which occurred likely based on the iron(II)-center-recycling with specific rate constant of 0.19 s^–1.

中文翻译：

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整体式肽-核酸杂合体，起着人工微过氧化物酶的作用

已经开发了具有安装的过氧化物酶功能的新的肽核酸（PNA）。Fmoc固相肽合成制备了呈现微过氧化物酶骨架的PNA杂种（VQKCAQCHTVE-（C ₂ H ₄ O）₂ CH _2- [PNA（T）] ₆ -G），然后用血红素重构。所得的全息化合物催化H ₂ O ₂氧化3,3'，5,5'-四甲基联苯胺到天然微过氧化物酶11的50％，而脱辅基形式和血红素则没有反应。发现过氧化物酶结构域对直接电化学具有活性，并且PNA杂合体用作基因传感器。在存在目标DNA（5'-CATGTATAAAAAA-3'）的情况下，带有电极的DNA探针（5'-TsTsTsTsTsTCTCATACATG-3'）显示了铁-亚铁准可逆波（-276 mV vs Ag / AgCl）通过夹心杂交。此外，杂交产物可以接受H ₂ O ₂作为氧化剂来增强还原电流，这可能是由于铁（II）中心循环，比速率常数为0.19 s ^–1所致。