Hydrophilic interaction liquid chromatography (HILIC) has gained widespread acceptance as an alternative to reversed-phase liquid chromatography (RPLC) for the retention and separation of polar compounds. Because a great number of emerging organic contaminants are polar, this represents progress in terms of solving most of the problems and limitations encountered in the conventional methods developed for their determination. In this review, recent HILIC applications developed in the environmental field are described, which are normally coupled with mass spectrometry, in order to reach the limits required to quantify contaminants present at trace levels and benefit from its capacity for confirmation. Special attention is paid to the stationary and mobile phases commonly used in terms of the advantages that they offer compared to reversed-phase columns and the high-water content mobile phases traditionally used in RPLC. The most important features related to the matrices and contaminants normally studied are also discussed, as well as the contribution of the HILIC mode towards reducing the matrix effect.

中文翻译：

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亲水相互作用液相色谱结合基于质谱的检测来确定环境样品中的新兴有机污染物

亲水相互作用液相色谱法（HILIC）作为反相液相色谱法（RPLC）的保留和分离极性化合物的替代方法已得到广泛认可。由于大量的新兴有机污染物是极性的，因此，这代表了在解决为确定传统方法而开发的常规方法中遇到的大多数问题和局限性方面取得的进步。在这篇综述中，描述了在环境领域开发的最新HILIC应用，通常将其与质谱联用，以达到量化痕量水平上存在的污染物所需的限度，并受益于其确认能力。特别要注意的是固定相和流动相相对于反相色谱柱和RPLC中传统使用的高含水量流动相相比具有的优势。还讨论了与通常研究的基质和污染物有关的最重要特征，以及HILIC模式对降低基质效应的贡献。