Ultrasensitive Detection of Protein with Wide Linear Dynamic Range Based on Core–Shell SERS Nanotags and Photonic Crystal Beads,ACS Sensors

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Ultrasensitive Detection of Protein with Wide Linear Dynamic Range Based on Core–Shell SERS Nanotags and Photonic Crystal Beads
ACS Sensors ( IF 8.2 ) Pub Date : 2017-07-18 00:00:00 , DOI: 10.1021/acssensors.7b00310
Bing Liu , Haibin Ni , Di Zhang , Delong Wang , Degang Fu , Hongyuan Chen ₁ , Zhongze Gu , Xiangwei Zhao

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Detection of proteins in a wide concentration range from fg mL^–1 to sub mg mL^–1 is a challenge in the high throughput analysis of precision medicine. Herein, we proposed a biosensor consisting of core–shell surface-enhanced Raman scattering (SERS) nanotags as labels and photonic crystal beads (PCBs) as carriers for ultrasensitive detection of proteins. In practice, Raman dyes (RDs) were embedded in the interface of gold core and silver shell in the bimetal nanoparticles to form SERS nanotags. It was found that the sensitivity was significantly improved due to the enhanced Raman signal by the coupling of the core–shell structure and linear dynamic range (LDR) was extended owing to the high surface to volume ratio of PCBs as well. In addition, we also demonstrated that the biosensor exhibited fine stability and low background, which has great application potential in the detection of protein biomarkers.

中文翻译：

基于核-壳SERS纳米标签和光子晶体珠的超宽动态范围蛋白质超灵敏检测

从fg mL ^–1到亚mg mL ^–1的宽浓度蛋白质检测在精密药物的高通量分析中是一个挑战。在本文中，我们提出了一种生物传感器，该传感器由核壳表面增强拉曼散射（SERS）纳米标签作为标签，以及光子晶体珠（PCB）作为载体，用于蛋白质的超灵敏检测。在实践中，拉曼染料（RDs）嵌入双金属纳米颗粒中金核和银壳的界面中，形成SERS纳米标签。研究发现，由于核壳结构的耦合，拉曼信号增强，灵敏度得到了显着提高，并且由于PCB的高体积比，线性动态范围（LDR）也得到了扩展。此外，我们还证明了该生物传感器表现出良好的稳定性和低背景，在蛋白质生物标志物的检测中具有巨大的应用潜力。

更新日期：2017-07-19

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