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Electrochromic Sensor for Multiplex Detection of Metabolites Enabled by Closed Bipolar Electrode Coupling
ACS Sensors ( IF 8.2 ) Pub Date : 2017-07-17 00:00:00 , DOI: 10.1021/acssensors.7b00292
Wei Xu 1 , Kaiyu Fu 1 , Paul W. Bohn 1
Affiliation  

Biosensors based on converting electrochemical signals into optical readouts are attractive candidates as low-cost, high-throughput sensor platforms. Here, we described a closed bipolar electrode (CBE)-based two-cell electrochromic device for sensing multiple metabolites, using the simultaneous detection of lactate, glucose, and uric acid as a model system. In the two-cell configuration, an analytical cell contains a redox mediator combined with a specific oxidase, e.g., lactate oxidase, glucose oxidase, or uricase, to form an electrochemical mediator–electrocatalyst pair that supports redox cycling. A closed bipolar electrode couples the electron transfer event in the analytical cell to an electrochromic reaction in a separate reporter cell, such that the magnitude of the color change is related to the concentration of metabolites in the analytical cell. To demonstrate multiplex operation, the CBE-based electrochromic detector is modified by integrating three sets of detection chemistries into a single device, in which simultaneous determination of glucose, lactate, and uric acid is demonstrated. Device sensitivity can be tuned by using reporter cells with different volumes. Furthermore, the analytical cell of this device can be fabricated as a disposable, paper-based carbon electrode without any pretreatment, demonstrating the potential to screen phenotypes that require multiple biomarkers in a point-of-care format.

中文翻译:

电致变色传感器用于代谢物封闭双极电极耦合的多重检测

基于低成本的高通量传感器平台,基于将电化学信号转换为光学读数的生物传感器是有吸引力的候选者。在这里,我们描述了一种基于封闭双极电极(CBE)的两细胞电致变色设备,用于同时检测乳酸,葡萄糖和尿酸作为模型系统,以检测多种代谢物。在两池配置中,分析池包含与特定氧化酶(例如乳酸氧化酶,葡萄糖氧化酶或尿酸酶)结合的氧化还原介体,以形成支持氧化还原循环的电化学介体-电催化剂对。封闭的双极电极将分析池中的电子转移事件与单独的报告池中的电致变色反应耦合,因此颜色变化的幅度与分析池中代谢物的浓度有关。为了演示多重操作,通过将三套检测化学试剂集成到一个设备中对基于CBE的电致变色检测器进行了修改,在该设备中,可同时测定葡萄糖,乳酸和尿酸。可以通过使用不同体积的报告单元来调节设备灵敏度。此外,该设备的分析池可被制成一次性的纸基碳电极,而无需任何预处理,从而证明了有可能以即时护理的形式筛选需要多种生物标记物的表型。其中可以同时测定葡萄糖,乳酸和尿酸。可以通过使用不同体积的报告单元来调节设备灵敏度。此外,该设备的分析池可被制成一次性的纸基碳电极,而无需任何预处理,从而证明了有可能以即时护理的形式筛选需要多种生物标记物的表型。其中可以同时测定葡萄糖,乳酸和尿酸。可以通过使用不同体积的报告单元来调节设备灵敏度。此外,该设备的分析池可被制成一次性的纸基碳电极,而无需任何预处理,从而证明了有可能以即时护理的形式筛选需要多种生物标记物的表型。
更新日期:2017-07-18
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