Translation is a critical process in protein synthesis, but translational regulation in antigen-specific T cells in vivo has not been well defined. Here we have characterized the translatome of virus-specific CD8+ effector T cells (Teff cells) during acute infection of mice with lymphocytic choriomeningitis virus (LCMV). Antigen-specific T cells exerted dynamic translational control of gene expression that correlated with cell proliferation and stimulation via the T cell antigen receptor (TCR). The translation of mRNAs that encode translation machinery, including ribosomal proteins, was upregulated during the T cell clonal-expansion phase, followed by inhibition of the translation of those transcripts when the CD8+ Teff cells stopped dividing just before the contraction phase. That translational suppression was more pronounced in terminal effector cells than in memory precursor cells and was regulated by antigenic stimulation and signals from the kinase mTOR. Our studies show that translation of transcripts encoding ribosomal proteins is regulated during the differentiation of CD8+ Teff cells and might have a role in fate 'decisions' involved in the formation of memory cells.

中文翻译：

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在CD8 +效应T细胞分化过程中，翻译受到积极调节。

翻译是蛋白质合成中的关键过程，但是体内抗原特异性T细胞的翻译调控尚未明确。在这里，我们表征了在淋巴细胞性脉络膜脑膜炎病毒（LCMV）小鼠急性感染过程中病毒特异性CD8 +效应T细胞（Teff细胞）的跨膜组。抗原特异性T细胞对基因表达进行动态翻译控制，与通过T细胞抗原受体（TCR）的细胞增殖和刺激相关。在T细胞克隆扩增阶段，编码翻译机制（包括核糖体蛋白）的mRNA的翻译被上调，随后当CD8 + Teff细胞在收缩阶段之前停止分裂时，这些转录物的翻译受到抑制。该翻译抑制在末端效应细胞中比在记忆前体细胞中更明显，并且受抗原刺激和来自激酶mTOR的信号调节。我们的研究表明，编码核糖体蛋白的转录本的翻译在CD8 + Teff细胞分化过程中受到调节，并且可能在参与记忆细胞形成的命运“决定”中发挥作用。