A series of Zr-based metal–organic framework (MOF) composites embedded with three kinds of aptamer strands (509-MOF@Apt) were achieved by a one-step de novo synthetic approach. A platform for ultrasensitive detection of analytes, namely, thrombin, kanamycin, and carcinoembryonic antigen (CEA), was also established. Considering the conformational changes caused by the binding interactions between aptamer strands and targeted molecules, the label-free electrochemical aptasensors based on 509-MOF@Apt composites could be developed to detect various target molecules. By comparing the common fabrication approaches of aptasensors, a distinct determination mechanism was presented through analysis of the electrochemical measurements on different interaction behaviors between probe aptamer strands and 509-MOF materials. The optimized aptasensors based on 509-MOFs@Apt demonstrated excellent sensitivity (with the detection limit of 0.40, 0.37, and 0.21 pg mL^–1 for CEA, thrombin, and kanamycin, respectively), stability, repeatability, and applicability. This work will provide a new platform for direct and feasible detection in biosensing related to clinical diagnostics and therapeutics, and further, extend the scope of potential applications for MOF materials.

中文翻译：

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由De Novo生物启发方法和增强的生物传感制备的适体嵌入锆基金属有机骨架复合材料，用于检测痕量分析物。

通过一步从头合成方法获得了一系列嵌入了三种适体链（509-MOF @ Apt）的Zr基金属-有机骨架（MOF）复合材料。还建立了用于超灵敏检测分析物（凝血酶，卡那霉素和癌胚抗原（CEA））的平台。考虑到适体链与目标分子之间的结合相互作用引起的构象变化，可以开发基于509-MOF @ Apt复合材料的无标记电化学适体传感器，以检测各种目标分子。通过比较适体传感器的常见制造方法，通过对探针适体链与509-MOF材料之间不同相互作用行为的电化学测量进行分析，提出了一种独特的确定机制。CEA，凝血酶和卡那霉素分别为^–1），稳定性，可重复性和适用性。这项工作将为直接和可行的与临床诊断和治疗相关的生物传感检测提供新的平台，并进一步扩展MOF材料的潜在应用范围。