当前位置: X-MOL 学术Annu. Rev. Anal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Bioanalytical Measurements Enabled by Surface-Enhanced Raman Scattering (SERS) Probes
Annual Review of Analytical Chemistry ( IF 5.9 ) Pub Date : 2017-06-12 00:00:00 , DOI: 10.1146/annurev-anchem-071015-041557
Lauren E. Jamieson 1 , Steven M. Asiala 1 , Kirsten Gracie 1 , Karen Faulds 1 , Duncan Graham 1
Affiliation  

Since its discovery in 1974, surface-enhanced Raman scattering (SERS) has gained momentum as an important tool in analytical chemistry. SERS is used widely for analysis of biological samples, ranging from in vitro cell culture models, to ex vivo tissue and blood samples, and direct in vivo application. New insights have been gained into biochemistry, with an emphasis on biomolecule detection, from small molecules such as glucose and amino acids to larger biomolecules such as DNA, proteins, and lipids. These measurements have increased our understanding of biological systems, and significantly, they have improved diagnostic capabilities. SERS probes display unique advantages in their detection sensitivity and multiplexing capability. We highlight key considerations that are required when performing bioanalytical SERS measurements, including sample preparation, probe selection, instrumental configuration, and data analysis. Some of the key bioanalytical measurements enabled by SERS probes with application to in vitro, ex vivo, and in vivo biological environments are discussed.

中文翻译:


表面增强拉曼散射(SERS)探针实现的生物分析测量

自1974年被发现以来,表面增强拉曼散射(SERS)作为分析化学的重要工具而获得了发展。SERS被广泛用于分析生物样品,从体外细胞培养模型到离体组织和血液样品,以及直接在体内的应用。从小分子(如葡萄糖和氨基酸)到较大的生物分子(如DNA,蛋白质和脂质),生物化学领域获得了新的见识,重点是生物分子的检测。这些测量增加了我们对生物系统的了解,并且显着地,它们具有提高的诊断能力。SERS探针在检测灵敏度和多路复用能力方面显示出独特的优势。我们着重介绍进行生物分析SERS测量时需要考虑的关键因素,包括样品制备,探针选择,仪器配置和数据分析。讨论了SERS探针在体外,离体和体内生物环境中应用所能实现的一些关键的生物分析测量。

更新日期:2017-06-12
down
wechat
bug