Annual Review of Analytical Chemistry ( IF 5.9 ) Pub Date : 2016-06-15 00:00:00 , DOI: 10.1146/annurev-anchem-071015-041627 Chien-Sheng Liao 1 , Ji-Xin Cheng 1
Coherent Raman scattering (CRS) microscopy is a high-speed vibrational imaging platform with the ability to visualize the chemical content of a living specimen by using molecular vibrational fingerprints. We review technical advances and biological applications of CRS microscopy. The basic theory of CRS and the state-of-the-art instrumentation of a CRS microscope are presented. We further summarize and compare the algorithms that are used to separate the Raman signal from the nonresonant background, to denoise a CRS image, and to decompose a hyperspectral CRS image into concentration maps of principal components. Important applications of single-frequency and hyperspectral CRS microscopy are highlighted. Potential directions of CRS microscopy are discussed.
中文翻译:
通过相干拉曼散射显微镜进行原位和体内分子分析
相干拉曼散射 (CRS) 显微镜是一种高速振动成像平台,能够利用分子振动指纹可视化活体样本的化学成分。我们回顾了 CRS 显微镜的技术进步和生物学应用。介绍了 CRS 的基本理论和最先进的 CRS 显微镜仪器。我们进一步总结和比较了用于将拉曼信号与非共振背景分离、对 CRS 图像进行降噪以及将高光谱 CRS 图像分解为主成分浓度图的算法。强调了单频和高光谱 CRS 显微镜的重要应用。讨论了 CRS 显微镜的潜在方向。